Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32164
Title: Production of yellow fever virus in microcarrier-based Vero cell cultures
Authors: Souza, Marta Cristina O.
Freire, Marcos S.
Schulze, Erica A.
Gaspar, Luciane P.
Castilho, Leda R.
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil.
Abstract: In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 × 108 pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.
Keywords: Yellow fever virus
Microcarrier-based culture
Vero cells
Serum-free medium
Issue Date: 2009
Citation: SOUZA, Marta Cristina O. et al. Production of yellow fever virus in microcarrier-based Vero cell cultures. Vaccine, Amsterdam, v. 27, n. 46, p. 6420-6423, 2009.
DOI: 10.1016/j.vaccine.2009.06.023
ISSN: 1873-2518
Copyright: restricted access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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