Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32184
Title: Simple immunoaffinity method to purify recombinant hepatitis B surface antigen secreted by transfected mammalian cells
Authors: Mouta Junior, Sergio da Silva e
Vianna, Carlos Otávio Alves
Ennes, Ilka
Gomes, Selma de Andrade
Freire, Marcos da Silva
Silva, Edimilson Domingos da
Simone, Salvatore Giovanni de
Moraes, Marcia Terezinha Baroni de
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Proteínas e Peptídeos, Bioquímica. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: Purification of recombinant hepatitis B surface antigen (recHBsAg) produced in a stable Chinese hamster ovary (CHO) cell line was evaluated using Linx Affinity Purification System (Invitrogen, USA). To purify HBsAg secreted by this cell line, a murine monoclonal antibody (MAbAH1) raised against native HBsAg was used. The purified AH1MAb was conjugated with phenyldiboronic acid (PDBA) and immobilized on the immunoaffinity chromatographic support. Using an optimized protocol the affinity column was able to purify recHBsAg from supernatant of mammalian cells cultures with more than 80% purity. This method showed to be simple and quicker than the current ultracentrifugation methods. The method is also efficient and economical in obtaining purified recHBsAg.
Keywords: Purification
Recombinant hepatitis B surface antigen
Issue Date: 2003
Citation: MOUTA JUNIOR, Sergio da Silva e et al. Simple immunoaffinity method to purify recombinant hepatitis B surface antigen secreted by transfected mammalian cells. Journal of Chromatography B, Amsterdam, v. 787, n. 2, p. 303-311, 2003.
DOI: 10.1016/S1570-0232(02)00954-6
ISSN: 1873-376X
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos

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