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https://www.arca.fiocruz.br/handle/icict/32266
DYNAMICS OF CONNECTIVE MATRIX DEPOSITION IN ACUTE EXPERIMENTAL E. COLI PYELONEPHRITIS IN RATS
Pielonefrite de fibrose
Tipos de colágeno
Mudanças matriciais
Matriz conectiva
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institute of Psychiatry. Department of Neuropahtology. London, England.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Bahia. Department of Medicine. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Bahia. Department of Medicine. Salvador, BA, Brazil.
Abstract
Adult male rats were subjected to pyelonephritis by direct kidney intramedullary injection of 0.1 ml saline suspension of 105 E. coli. Animals were killed at intervals of 4, 10, 15, 30 and 60 days. Half of each kidney and bladder were cultured in proper bacteriologic media to demonstrate
the existence of infection. The other halves were submitted to light microscopy and ultrastructural studies. Immunofluorescence methods were used for the study of connective matrix components, at the initial stage of the inflammatory process (4, 10 and 15 days). Infection was documented by bacteriologic, gross and microscopic findings in all groups following inoculation, and it lasted up to two months. Following the acute inflammatory reaction, fibronectin and type III collagen were deposited
in the interstitium of kidneys. Small amounts of type I collagen were found later. Type IV collagen appeared in small quantities, associated with collapse of structures containing basement membranes. Fibronectin became concentrated in re-activated foci. The subsequent scarring
and associated focal renal atrophy depended upon the extension of the acute lesion.
Keywords in Portuguese
PielonefritePielonefrite de fibrose
Tipos de colágeno
Mudanças matriciais
Matriz conectiva
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