Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32292
Title: Resialylation of sialidase-treated sheep and human erythrocytes by Trypanosoma cruzi trans-sialidase: restoration of complement resistance of desialylated sheep erythrocytes
Authors: Tomlinson, Stephen
Pontes-de-Carvalho, Lain Carlos
Vandekerckhove, Filip
Nussenzweig, Victor
Affilliation: New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
Abstract: Trypanosoma cruzi trans-sialidase (TS) is a recently described enzyme whkh transfers a(2-3)-linked sialic acid from host-derived sialylated glycoconjugates to parasite surface molecules [Schenkman et al. (1991) Cell, 65, 1117]. We report here on the ability of TS to transfer sialic acid from donor sialyl-a(2-3)lactose to sialidase-treated sheep and human erythrocytes. Up to ~ 50% resialylation of both desialylated red cells could be attained. Resialylation of desialylated sheep erythrocytes restores their resistance to lysis by human complement. This ascribes a possible biological role for T.cruzi TS and demonstrates directly that sialic acid is solely responsible for preventing alternative pathway activation of human complement by sheep erythrocytes.
Keywords: Trypanosoma cruzi
Erythrocytes
Complement
Acid sialidase
Sialic acid
Trans-sialidase
keywords: Trypanosoma cruzi
Eritrócitos
Complemento
Ácido sialidase
Acido sialico
Trans-sialidase
Issue Date: 1992
Publisher: Oxford University Press
Citation: TOMLINSON, S. et al. Resialylation of sialidase-treated sheep and human erythrocytes by Trypanosoma cruzi trans-sialidase: restoration of complement resistance of desialylated sheep erythrocytes. Glycobiology, v. 2, n. 6, p. 549-551, 1992.
Description: Pontes-de-Carvalho, Lain Carlos. Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
ISSN: 0959-6658
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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