Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32343
Title: Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA)
Authors: Thomé, Sabrina
Lessa-Aquino, Carolina
Ko, Albert Icksang
Lilenbaum, Walter
Medeiros, Marco Alberto
Affilliation: Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Yale University. School of Public Health, Department of Epidemiology of Microbial Diseases. Downtown, New Haven, USA.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: BACKGROUND: The microscopic agglutination test (MAT), the standard method for serological diagnosis of leptospirosis, may present limitations regarding its sensitivity. Current studies suggest that Leptospira immunoglobulin-like (Lig) proteins and LipL32 are of particular interest as serodiagnostic markers since they are present only in pathogenic species of the Leptospira genus. The purpose of this study was to identify leptospiral immunodominant proteins that are recognized by canine sera from diseased dogs. RESULTS: A total of 109 dogs were studied, including seroreactive dogs (MAT ≥800) and dogs with no seroreactivity detectable by MAT. Eight recombinant fragments (31-70 kDa) of pathogenic Leptospira were tested for their use as diagnostic markers for canine leptospirosis using the Multi-antigen Print Immunoassay (MAPIA) platform: LigB [582-947aa] from L. interrogans serovar Pomona, L. interrogans serovar Copenhageni and L. kirschneri serovar Gryppotyphosa, LigB [131-649aa] from L. interrogans serovar Copenhageni, L. interrogans serovar Canicola and L. kirschneri serovar Gryppotyphosa, LigA [625-1224aa] L. interrogans serovar Copenhageni and LipL32 from L. interrogans serovar Copenhageni. The data were analyzed and ROC curves were generated. Altogether, LigB [131-649aa] L. interrogans Canicola, LigB [131-649aa] L. kirschneri Gryppotyphosa and LipL32 L. interrogans Copenhageni showed best accuracy (AUC = 0.826 to 0.869), with 70% specificity and sensitivity ranging from 89% to 95%. CONCLUSIONS: These results reinforce their potential as diagnostic candidates for the development of new methods for the serological diagnosis of canine leptospirosis.
Keywords: Leptospirosis
Dogs
MAPIA
LipL32
Lig proteins
Issue Date: 2014
Citation: THOMÉ, Sabrina et al. Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA). Bmc Veterinary Research, London, v. 288, n. 10, p. 2-9, 2014.
DOI: 10.1186/s12917-014-0288-2
ISSN: 1746-6148
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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