Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32344
Title: The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model
Authors: Lucena, Alessandra M. M.
Souza, Cássio R. M.
Jales, Jéssica T.
Guedes, Paulo M. M.
Miranda, George E. C. de
Moura, Adolpho M. A. de
Araújo-Júnior, João X.
Nascimento, George J.
Scortecci, Kátia C.
Santos, Barbara V. O.
Souto, Janeusa T. Souto
Affilliation: Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Lagoa Nova, Natal, RN, Brasil.
Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Lagoa Nova, Natal, RN, Brasil.
Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Lagoa Nova, Natal, RN, Brasil.
Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Lagoa Nova, Natal, RN, Brasil.
Universidade Federal da Paraíba. Departamento de Sistemática e Ecologia. Laboratório de Algas Marinhas. João Pessoa, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Alagoas. Escola de Enfermagem e Farmácia. Maceió, Brasil.
Universidade Federal de Campina Grande. Unidade Acadêmica de Ciências Biológicas. Patos, Brasil.
Universidade Federal do Rio Grande do Norte. Departamento de Biologia Celular e Genética. Campus Universitário, Lagoa Nova, Natal, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. Programa de Pós-Graduação em Produtos Naturais e Bioativos Sintéticos. João Pessoa, PB, Brasil.
Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Lagoa Nova, Natal, RN, Brasil.
Abstract: Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze the effect of CLP in the murine model of peritonitis and ulcerative colitis. Firstly, the mice were submitted to peritonitis to evaluate which dose of CLP (40, 4, or 0.4 mg/kg) could decrease the inflammatory infiltration in the peritoneum. The most effective doses were 40 and 4 mg/kg. Then, C57BL/6 mice were submitted to colitis development with 3% dextran sulfate sodium (DSS) and treated with CLP at doses of 40 and 4 mg/kg. The disease development was analyzed through the disease activity index (DAI); furthermore, colonic tissue samples were submitted to histological analysis, NFκB determination, and in vitro culture for cytokines assay. Therefore, CLP at 4 mg/kg presented the best results, triggering improvement of DAI and attenuating the colon shortening and damage. This dose was able to reduce the TNF-α, IFN-γ, IL-6, IL-17, and NFκB p65 levels, and increased the levels of IL-10 in the colon tissue. Thus, CLP mice treatment at a dose of 4 mg/kg showed promising results in ameliorating the damage observed in the ulcerative colitis.
Keywords: Caulerpin
Ulcerative colitis
Anti-inflammatory activity
Cytokines
Tissue damage
Issue Date: 2018
Citation: LUCENA, Alessandra M. M. et al. The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model. Marine Drugs, Basel, v. 16, n. 9, p. 2-18, 2018.
DOI: 10.3390/md16090318
ISSN: 1660-3397
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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