Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32347
Title: Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product
Authors: Bastos, Renata Chagas
Carvalho, Juliana Machado de
Silveira, Ivna Alana Freitas Brasileiro da
Jacob, Silvana do Couto
Leandro, Katia Christina
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ. Brasil.
Abstract: In Brazil, polysaccharide–protein conjugate vaccine against Neisseria meningitidis group C (MenCPS-TT) using hydrazine-activated-tetanus toxoid (TT) as a carrier protein has been developed. Because of the toxicity of hydrazine in humans, it is necessary to monitor this substance's process control step during the vaccine production. The electroanalytical methodology was developed and validated for the determination of hydrazine during the process control of MenCPS-TT vaccine production by differential pulse polarography. The reduction potential was −0.95 V in acetone and sulphuric acid solution. The method presented linear range between 30 and 150 μg L−1and recovery of 93.5 ± 0.8%.
Keywords: Hydrazine
Meningococcal vaccine
Polarography
Issue Date: 2010
Citation: BASTOS, Renata Chagas et al. Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product. Vaccine, Amsterdam, v. 28, n. 34, p. 5648-5651, 2010.
DOI: 10.1016/j.vaccine.2010.05.079
ISSN: 1873-2518
Copyright: restricted access
Appears in Collections:INCQS - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos

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