Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32399
Title: Accuracy and repeatability of a micro plaque reduction neutralization test for vaccinia antibodies
Authors: Borges, Maria Beatriz J.
Kato, Sayuri E.M.
Damaso, Clarissa R. A.
Moussatché, Nissin
Freire, Marcos da Silva
Passos, Sonia Regina Lambert
Nascimento, Jussara Pereira do
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia Molecular de Vírus. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia Molecular de Vírus. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia Molecular de Vírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: The detection of neutralizing antibodies against vaccinia virus is a valuable tool for the investigation of previous smallpox vaccination. Compulsory smallpox vaccination ended in Brazil during the early 1970s, although the vaccine was available until the late 1970s. The threat of smallpox as a biological weapon has called the attention of public health authorities to the need for an evaluation of the immune status of the population. Based on our previous experience with a micro plaque reduction neutralization test (PRNT) for the evaluation of yellow fever immunity, a similar test was developed for the detection and quantification of vaccinia neutralizing antibodies. A cross-sectional study to test the repeatability and validity of plaque reduction neutralization test (PRNT) for vaccinia antibodies was performed in 182 subjects divided into two categories: subjects above 31 years old and the other ≥35 years old. Cases were subjects considered to have been vaccinated with vaccinia virus if they declared vaccination history or evidenced vaccination marks. The assay is carried out in 96-well plates, provides results within 30 h, is easily performed, has good sensitivity (92.7%) and specificity (90.8), excellent repeatability (ICC 0.89 (0.88; 0.92)) and is thus suitable for use in mass screening of a population's antibody levels.
Keywords: Smallpox
Microneutralization test
Plaque reduction
International Standard Serum
Issue Date: 2008
Citation: BORGES, Maria Beatriz J. et al. Accuracy and repeatability of a micro plaque reduction neutralization test for vaccinia antibodies. Biologicals, San Diego, v. 36, n. 2, p. 105-110, 2008.
DOI: 10.1016/j.biologicals.2007.07.001
ISSN: 1095-8320
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos

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