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ISOLATION OF LEPTOSPIRA INTERROGANS HARDJOPRAJITNO FROM VAGINAL FLUID OF A CLINICALLY HEALTHY EWE SUGGESTS POTENTIAL FOR VENEREAL TRANSMISSION
Director, A. et al. | Date Issued: 2014
Author
Director, A.
Penna, B.
Hamond, C.
Loureiro, A. P.
Martins, G.
Medeiros, M. A.
Lilenbaum, W.
Affilliation
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Abstract
A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of Leptospira sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the rrs and secY genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as Leptospira interrogans serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable Leptospira in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.
Keywords
Leptospira interrogans Hardjoprajitno
Vaginal fluid
Clinically healthy
Venereal transmission
 
Citation
DIRECTOR, A. et al. Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission. Journal of Medical Microbiology, London, v. 63, p. 1234-1236, 2014.
DOI
10.1099/jmm.0.065466-0
ISSN
1473-5644