Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32455
Title: Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
Authors: Dopico, Eva
Del-Rei, Rodrigo Pimenta
Espinoza, Bertha
Ubillos, Itziar
Zanchin, Nilson Ivo Tonin
Sulleiro, Elena
Moure, Zaira
Celedon, Paola Alejandra Fiorani
Souza, Wayner Vieira
Silva, Edimilson Domingos da
Gomes, Yara Miranda
Santos, Fred Luciano Neves
Affilliation: Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain.
Faculty of Technology and Sciences of Bahia. Salvador, Bahia, Brazil.
Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas. Departamento de Inmunología. Ciudad de México, Mexico.
Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain.
Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain.
Institute of Paraná. Molecular Biology. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Methods: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. Results: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. Conclusions: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in nonendemic settings.
Keywords: Chagas disease
Trypanosoma cruzi
Chimeric antigens
Immunoassay
Accuracy
keywords: Doença de Chagas
Trypanosoma cruzi
Antígenos Quiméricos
Imunoensaio
Precisão
Issue Date: 2019
Publisher: BMC
Citation: DOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019.
DOI: 10.1186/s12879-019-3872-z
ISSN: 1471-2334
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
PE - IAM - Artigos de Periódicos
PR - ICC - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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