Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32472
Title: Pressure-inactivated yellow fever 17DD virus: implications for vaccine development
Authors: Gaspar, Luciane P.
Mendes, Ygara S.
Yamamura, Anna M. Y.
Almeida, Luiz F. C.
Caride, Elena
Gonçalves, Rafael B.
Silva, Jerson L.
Oliveira, Andréa C.
Galler, Ricardo
Freire, Marcos S.
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: The successful Yellow Fever (YF) vaccine consists of the live attenuated 17D-204 or 17DD viruses. Despite its excellent record of efficacy and safety, serious adverse events have been recorded and influenced extensive vaccination in endemic areas. Therefore, alternative strategies should be considered, which may include inactivated whole virus. High hydrostatic pressure has been described as a method for viral inactivation and vaccine development. The present study evaluated whether high hydrostatic pressure would inactivate the YF 17DD virus. YF 17DD virus was grown in Vero cells in roller bottle cultures and subjected to 310 MPa for 3 h at 4 °C. This treatment abolished YF infectivity and eliminated the ability of the virus to cause disease in mice. Pressure-inactivated virus elicited low level of neutralizing antibody titers although exhibited complete protection against an otherwise lethal challenge with 17DD virus in the murine model. The data warrant further development of pressure-inactivated vaccine against YF.
Keywords: Yellow fever 17DD virus
High pressure
Virus inactivation
Issue Date: 2008
Citation: GASPAR, Luciane P. et al. Pressure-inactivated yellow fever 17DD virus: Implications for vaccine development. Journal of Virological Method, Amsterdam, v. 150, n. 1-2, p. 57-62, 2008.
DOI: 10.1016/j.jviromet.2008.03.002
ISSN: 1879-0984
Copyright: restricted access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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