Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32487
Title: Biological characterization of clones derived from the Edmonston strain of measles virus in comparison with Schwarz and CAM-70 vaccine strains
Authors: Borges, Maria Beatriz Junqueira
Mann, George F.
Freire, Marcos da Silva
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: Four virus clones were derived from the Edmonston strain of measles virus by repeated plaque purification. These clones were compared with the vaccine strains Schwarz and CAM-70 in terms of biological activities including plaque formation, hemagglutination, hemolysis and replication in Vero cells and chick embryo fibroblasts (CEF). Two clones of intermediate plaque yielded mixed plaque populations on subcultivation whereas the other two, showing small and large plaque sizes, showed stable plaque phenotypes. The vaccine strains showed consistent homogeneous plaque populations. All the Edmonston clones showed agglutination of monkey erythrocytes in isotonic solution while both vaccine strains hemagglutinated only in the presence of high salt concentrations. Variation in the hemolytic activity was observed among the four clones but no hemolytic activity was detected for the vaccine virus strains. Vaccine strains replicated efficiently both in Vero cells and CEF. All four clones showed efficient replication in Vero cells but different replication profiles in CEF. Two of them replicated efficiently, one was of intermediate efficiency and the other showed no replication in CEF. Two of the clones showed characteristics similar to vaccine strains. One in terms of size and homogeneity of plaques, the other for a low hemolytic activity and both for the efficiency of propagation in CEF.
Keywords: Biological characterization
Plaque variants
Measles
Vaccine
Issue Date: 1996
Citation: BORGES, Maria Beatriz Junqueira; MANN, George F; FREIRE, Marcos da Silva. Biological characterization of clones derived from the edmonston strain of measles virus in comparison with schwarz and CAM-70 vaccine strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 4, p. 507-514, 1996.
DOI: 10.1590/S0074-02761996000400020
ISSN: 1678-8060
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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