Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3253
Title: Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
Authors: Borges, Valeria de Matos
Lopes, Ulisses Gazos
Souza, Wanderley de
Santos, Marcos André Vannier dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: Multidrug-resistant Leishmania (Leishmania) amazonensis may be obtained by in vitro selection with vinblastine. In order to determine whether this phenotype is linked to structural alterations, we analyzed the cell architecture by electron microscopy. The vinblastine resistant CL2 clone of L. (L.) amazonensis, but not wild-type parasites, showed a cytokinesis dysfunction. The CL2 promastigotes had multiple nuclei, kinetoplasts and flagella, suggesting that vinblastine resistance may be associated with truncated cell division. The subpellicular microtubule plasma membrane connection was also affected. Wild-type parasites treated with vinblastine displayed similar alterations, presenting lobulated and multinucleated cells. Taken together, these data indicate that antimicrotubule drug-selected parasites may show evidence of the mutation of cytoskeleton proteins, impairing normal cell function.
DeCS: Leishmania mexicana
Resistência a Múltiplos Medicamentos
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Vimblastina
Citoesqueleto
Citocinese
Animais
Issue Date: 2004
Citation: BORGES, V. de M. et al. Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis. Parasitology Research, v.95, n.2, p.90-96, 2005.
DOI: 10.1007/s00436-004-1248-8
ISSN: 0932-0113
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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