Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32565
Title: Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
Authors: Alves, Gleica Soyan Barbosa
Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Pinheiro, José Felipe de Souza
Souza, João Vicente Braga de
Wanke, Bodo
Matsuura, Takeshi
Jackisch-Matsuura, Ani Beatriz
Affilliation: Universidade Federal do Amazonas. Programa de Pós-Graduação em Saúde Sociedade e Endemias na Amazônia. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus, AM, Brasil.
Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.
Centro de Controle de Zoonoses de Manaus. Manaus, AM, Brasil.
Instituto Nacional de Pesquisas da Amazonia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Amazonas. Laboratório de Microbiologia. Manaus, AM, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus, AM, Brasil.
Abstract: Cryptococcus neoformans and Cryptococcus gattii are the main causative agents of cryptococcosis, a systemic fungal disease that affects internal organs and skin, and which is acquired by inhalation of spores or encapsulated yeasts. It is currently known that the C. neoformans/C. gattii species complex has a worldwide distribution, however, some molecular types seem to prevail in certain regions. Few environmental studies of Cryptococcus have been conducted in the Brazilian Amazon. This is the first ecological study of the pathogenic fungi C. neoformans/C. gattii species complex in the urban area of Manaus, Amazonas, Brazil. A total of 506 samples from pigeon droppings (n = 191), captive bird droppings (n = 60) and tree hollows (n = 255) were collected from June 2012 to January 2014 at schools and public buildings, squares, pet shops, households, the zoo and the bus station. Samples were plated on niger seed agar (NSA) medium supplemented with chloramphenicol and incubated at 25°C for 5 days. Dark-brown colonies were isolated and tested for thermotolerance at 37°C, cycloheximide resistance and growth on canavanine-glycine-bromothymol blue agar. Molecular typing was done by PCR-RFLP. Susceptibility to the antifungal drugs amphotericin B, fluconazole, itraconazole and ketoconazole was tested using Etest(®) strips. In total, 13 positive samples were obtained: one tree hollow (C. gattiiVGII), nine pigeon droppings (C. neoformansVNI) and three captive bird droppings (C. neoformansVNI). The environmental cryptococcal isolates found in this study were of the same molecular types as those responsible for infections in Manaus.
Keywords: Cryptococcus neoformans
Cryptococcus gattii
Environmental isolation
Genotyping
Manaus
Amazonas
Brazil
Issue Date: 2016
Publisher: Wiley
Citation: ALVES, Gleica Soyan Barbosa et al. Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil. Mycoses, v. 59, p. 509-515, 2016.
DOI: 10.1111/myc.12499
ISSN: 0933-7407
Copyright: restricted access
Appears in Collections:AM - ILMD - Artigos de Periódicos
INI - Artigos de Periódicos

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