Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3263
Title: The modelling of mononuclear phagocyte-connective tissue adhesion in vitro: application to disclose a specific inhibitory effect of Leishmania infection
Authors: Carvalhal, Djalma Gomes Ferrão
Barbosa Junior, Aryon de Almeida
Hermida, Micely D' El Rei
Clarêncio, Jorge
Pinheiro Junior, Nathanael F
Veras, Patrícia Sampaio Tavares
dosSantos, Washington Luis Conrado
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: In this work, we have developed an adhesion assay to study interactions between mononuclear phagocytes and connective tissue in vitro and show its potential use to study diseases caused by intracellular microorganisms. The assay reproduces most of the characteristics of macrophage adhesion to connective tissue in vivo, such as: preferential adhesion to inflamed connective tissue, divalent cation and integrin dependence, and up-regulation upon cell activation. The phagocyte adhesion to connective tissue was inhibited by infection with Leishmania (58+/-22%, p < 0.05) and was not affected by infection with Mycobacterium or by endocytosis of latex beads. Manganese partially reverted the loss in adherence produced by Leishmania infection, indicating that the mechanisms regulating the function of integrins are affected by cell infection with Leishmania. This assay might be a useful tool for the study of the mechanisms by which mononuclear phagocytes play a role in the immune-inflammatory response and in the development of lesions.
Keywords: Adhesion assay
Cell adhesion
Macrophage adhesion
J774 cells
Leishmania amazonensis
Leishmania braziliensis
Promastigotes
DeCS: Análise de Variância
Antígenos CD
Cátions Bivalentes
Adesão Celular
Linhagem Celular
Tecido Conjuntivo
Técnicas de Cultura
Inflamação
Integrinas
Leishmanias
Leucócitos Mononucleares
Manganês
Mycobacterium fortuitum
Fagócitos
Organismos Livres de Patógenos Específicos
Camundongos
Issue Date: 2004
Citation: CARVALHAL, D.G. et al. The modelling of mononuclear phagocyte-connective tissue adhesion in vitro: application to disclose a specific inhibitory effect of Leishmania infection. Experimental Parasitology, v. 107, n. 3-4, p. 189-199, jul.-aug. 2004.
DOI: 10.1016/j.exppara.2004.06.003
ISSN: 0014-4894
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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