Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32682
Title: Detection of IgG3 antibodies specific to the human immunodeficiency virus type 1 (HIV-1) p24 protein as marker for recently acquired infection
Authors: Viana, I. F. T.
Coêlho, D. F.
Palma, M. L.
Nascimento, E. J. M.
Gu, G.
Lima, L. F. O.
Foti, L.
Krieger, M. A.
Pilcher, C.
Calzavara-Silva, C. E.
Mailliard, R. B.
Rinaldo, C. R.
Dhalia, R.
Marques, E. T. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. USA / University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Pennsylvania‎, USA / Federal University of Pernambuco. Fundamental Chemistry Department. Recife, PE, Brazil.
University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
University of Pittsburgh. Center for Vaccine Research. USA / University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
University of Pittsburgh. Center for Vaccine Research. USA / Tsinghua University. School of Medicine. Beijin, China.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Paraná, Brasil / Technological Institute of Paraná/TECPAR. Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Paraná, Brasil / Molecular Biology Institute of Paraná/IBMP. Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Paraná, Brasil / Molecular Biology Institute of Paraná/IBMP. Brazil.
UCSF School of Medicine. USA.
Fundação Oswaldo Cruz. Instituto René Rachou. Minas Gerais, Brasil.
University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Pennsylvania‎, USA / University of Pittsburgh. Infectious Diseases and Microbiology Department. Pennsylvania‎, USA.
Abstract: Reducing the risk of human immunodeficiency virus type 1 (HIV-1) transmission is still a public health priority. The development of effective control strategies relies on the quantification of the effects of prophylactic and therapeutic measures in disease incidence. Although several assays can be used to estimate HIV incidence, these estimates are limited by the poor performance of these assays in distinguishing recent from long-standing infections. To address such limitation, we have developed an assay to titrate p24-specific IgG3 antibodies as a marker of recent infection. The assay is based on a recombinant p24 protein capable to detect total IgG antibodies in sera using a liquid micro array and enzyme-linked immunosorbent assay. Subsequently, the assay was optimised to detect and titrate anti-p24 IgG3 responses in a panel of sequential specimens from seroconverters over 24 months. The kinetics of p24-specific IgG3 titres revealed a transient peak in the 4 to 5-month period after seroconversion. It was followed by a sharp decline, allowing infections with less than 6 months to be distinguished from older ones. The developed assay exhibited a mean duration of recent infection of 144 days and a false-recent rate of ca. 14%. Our findings show that HIV-1 p24-specific IgG3 titres can be used as a tool to evaluate HIV incidence in serosurveys and to monitor the efficacy of vaccines and other transmission control strategies.
Keywords: ELISA
Early infection marker
HIV/AIDS
recent infection
DeCS: Anticorpos virais / sangue
Biomarcadores / sangue
Ensaio de Imunoadsorção Enzimática
Proteína Essencial do HIV p24 / imunologia
Infecções por HIV / diagnóstico
Infecções por HIV / epidemiologia
HIV-1 / imunologia
Humanos
Imunoglobulina G / sangue
Incidência
Cinética
Seroconversão
Estudos soroepidemiológicos
Fatores de Tempo
Issue Date: 2018
Citation: VIANA, I. F. T. et al. Detection of IgG3 Antibodies Specific to the Human Immunodeficiency Virus Type 1 (HIV-1) P24 Protein as Marker for Recently Acquired Infection. Epidemiology and Infection, v. 146, n. 10, p. 1293-1300, 2018.
DOI: 10.1017/S0950268818001218
ISSN: 1469-4409
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos
PR - ICC - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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