Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3271
Title: A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
Authors: Assis, Bárbara Cristina Alves de
Cunha, Liliane Monteiro
Baptista, Ana Paula
Andrade, Zilton de Araújo
Affilliation: Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: A highly specific pattern of immunofluorescence was noted when sera from Capillaria hepatica-infected rats were tested against the homologous worms and eggs present either in paraffin or cryostat sections from mouse liver. The pattern was represented by a combined apple green fluorescence of the internal contents of worms and eggs, which persisted in serum-dilutions of 1:400 up to 1:1600. Unequivocal fluorescent pattern was observed from 15 days up to 3 months following inoculation of rats with embryonated C. hepatica eggs and such result was confirmed by the ELISA. After the 4th month of infection, the indirect immunofluorescence test turned negative, probably revealing the extinction of parasitism, however the ELISA was contradictory, disclosing high levels of antibodies in this period . The IIF was also negative when control normal rat sera and sera from rats administered by gavage with immature C. hepatica eggs (spurious infection), or for reactions made against Schistosoma mansoni eggs, although a weakly positive pattern occurred with Fasciola hepatica eggs. The indirect immunofluorescence test may be recommended for use with human sera to detect early C. hepatica infection in special clinical instances and in epidemiological surveys, since it is a simple, inexpensive, and reliable test, presenting excellent sensitivity and specificity. Although the diagnosis is positive only during early infection,
Keywords: Capillaria hepatica
Immunofluorescence test
Rat Capillariasis
DeCS: Animais
Anticorpos Anti-Helmínticos
Capillaria
Infecções por Enoplida
ELISA
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Hepatopatias Parasitárias
Masculino
Camundongos
Contagem de Ovos de Parasitas
Ratos
Ratos Wistar
Sensibilidade e Especificidade
Issue Date: 2004
Citation: ASSIS, B.C. et al. A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test. Memórias do Instituto Oswaldo Cruz, v. 99, n. 2, p. 173-177, mar. 2004.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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