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https://www.arca.fiocruz.br/handle/icict/32715
YEAST-DERIVED BIOSYNTHESIS OF SILVER/SILVER CHLORIDE NANOPARTICLES AND THEIR ANTIPROLIFERATIVE ACTIVITY AGAINST BACTERIA
Yeasts
Metallic nanoparticles
Biomimetic synthesis
Silver/silver chloride nanoparticles
Author
Affilliation
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Federal University of Rio de Janeiro. Laboratory of Pharmaceutical Biotechnology. Rio de Janeiro, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
Laboratory of Biomineralization, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Federal University of Rio de Janeiro. Laboratory of Pharmaceutical Biotechnology. Rio de Janeiro, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
Laboratory of Biomineralization, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.
National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.
Abstract
Here, we provided the first evidences of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-Vis supported the biosynthesis. TEM analysis evidenced that nanoparticles mainly presented circular shape and their diameter varied mostly in the range from 2 to 10 nm. XRD analysis showed a crystalline structure, with diffraction peaks corresponding to metallic silver and silver chloride nanoparticles, and when analyzed by high-resolution transmission electron microscopy (HRTEM), instead of being round, (111) (octahedral) and (200) (cubic-) symmetry facets appeared systematically in one side of the nanoparticles. Analysis of ultra-thin sections by TEM indicated that the domain of the synthesis of Ag/AgCl-NPs were mainly between cell wall and the plasma membrane. By using 3D reconstruction obtained from focused ion beam scanning electron microscopy (FIB/SEM) the spatial distribution of the domains of nanoparticles synthesis was mapped and nanoaggregates of Ag/AgCl-NPs up 35 nm in diameter were observed. Extracellular synthesis also occurred; in accordance with the fact that conditioned media from yeast isolates were as efficient at producing Ag/AgCl-NPs as live-cell cultures. Exposure of Gram-positive Staphylococcus aureus and Gram-negative Klebsiella pneumoniae cultures to Ag/AgCl-NPs led to a strong growth inhibition as shown by optical density measurements. The Ag/AgCl-NPs described here have characteristics compatible with a strong potential for use in the biotechnology industry, particularly for biomedical applications.
Keywords
BiotechnologyYeasts
Metallic nanoparticles
Biomimetic synthesis
Silver/silver chloride nanoparticles
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