Author | Marcon, Bruna Hilzendeger | |
Author | Shigunov, Patrícia | |
Author | Spangenberg, Lucia | |
Author | Pereira, Isabela Tiemy | |
Author | Aguiar, Alessandra Melo de | |
Author | Amorín, Rocío | |
Author | Rebelatto, Carmen Lúcia Kuniyoshi | |
Author | Dominguez, Alejandro Correa | |
Author | Dallagiovanna, Bruno | |
Access date | 2019-04-30T18:19:40Z | |
Available date | 2019-04-30T18:19:40Z | |
Document date | 2019 | |
Citation | MARCON, Bruna H. et al. Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. Scientific Reports, v. 9, n. 5611, p. 1-10, 2019. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32891 | |
Language | por | pt_BR |
Publisher | Nature Research | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Perfil Polissômico | pt_BR |
Title | Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-019-42005-3 | |
Abstract | The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and to the accumulation of lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation of translational activity with a decrease in cell size, proliferation and migration. In the present study, we identified that after 24 hours of adipogenic induction, human adipose tissue-derived stem cells (hASCs) undergo a G1-cell cycle arrest consistent with reduced proliferation, and this effect was correlated with a shift in polysome profile with an enrichment of the monosomal fraction and a reduction of the polysomal fraction. Polysome profiling analysis also revealed that this change in the monosomal/polysomal ratio was related to a strong downregulation of cell cycle and proliferation genes, such as cyclins and cyclin-dependent kinases (CDKs). Comparing total and polysome-associated mRNA sequencing, we also observed that this downregulation was mostly due to a reduction of cell cycle and proliferation transcripts via control of total mRNA abundance, rather than by translational control. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | Stem Cells | pt_BR |
Subject | Adipogenesis | pt_BR |
Subject | Polyribosomes | pt_BR |
Subject | RNA, Messenger | pt_BR |
Subject | Down-Regulation | pt_BR |
Subject in Spanish | Células Madre | pt_BR |
Subject in Spanish | Adipogénesis | pt_BR |
Subject in Spanish | Polirribosomas | pt_BR |
Subject in Spanish | ARN Mensajero | pt_BR |
Subject in Spanish | Regulación hacia Abajo | pt_BR |
DeCS | Células-Tronco | pt_BR |
DeCS | Adipogenia | pt_BR |
DeCS | Polirribossomos | pt_BR |
DeCS | RNA Mensageiro | pt_BR |
DeCS | Regulação para Baixo | pt_BR |
e-ISSN | 2045-2322 | |