Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32891
Title: Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
Authors: Marcon, Bruna H.
Shigunov, Patrícia
Spangenberg, Lucia
Pereira, Isabela Tiemy
Aguiar, Alessandra Melo de
Amorín, Rocío
Rebelatto, Carmen K.
Correa, Alejandro
Dallagiovanna, Bruno
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.
Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract: The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and to the accumulation of lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation of translational activity with a decrease in cell size, proliferation and migration. In the present study, we identified that after 24 hours of adipogenic induction, human adipose tissue-derived stem cells (hASCs) undergo a G1-cell cycle arrest consistent with reduced proliferation, and this effect was correlated with a shift in polysome profile with an enrichment of the monosomal fraction and a reduction of the polysomal fraction. Polysome profiling analysis also revealed that this change in the monosomal/polysomal ratio was related to a strong downregulation of cell cycle and proliferation genes, such as cyclins and cyclin-dependent kinases (CDKs). Comparing total and polysome-associated mRNA sequencing, we also observed that this downregulation was mostly due to a reduction of cell cycle and proliferation transcripts via control of total mRNA abundance, rather than by translational control.
Keywords: Stem Cells
Adipogenesis
Polyribosomes
RNA, Messenger
Down-Regulation
Keywords in spanish: Células Madre
Adipogénesis
Polirribosomas
ARN Mensajero
Regulación hacia Abajo
keywords: Perfil Polissômico
DeCS: Células-Tronco
Adipogenia
Polirribossomos
RNA Mensageiro
Regulação para Baixo
Issue Date: 2019
Publisher: Nature Research
Citation: MARCON, Bruna H. et al. Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. Scientific Reports, v. 9, n. 5611, p. 1-10, 2019.
DOI: 10.1038/s41598-019-42005-3
ISSN: 2045-2322
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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