Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32943
Title: Identification of 170 new long noncoding RNAs in Schistosoma mansoni
Authors: Oliveira, Victor Fernandes de
Moraes, Lauro Ângelo Gonçalves de
Mota, Ester Alves
Passos, Liana Konovaloff Jannotti
Coelho, Paulo Marcos Zech
Mattos, Ana Carolina Alves de
Couto, Flávia Fernanda Búbula
Caffrey, Brian E.
Marsico, Annalisa
Cota, Renata Guerra de Sá
Affilliation: Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brasil.
Universidade Federal de Ouro Preto. Laboratório de Computação de Sistemas Inteligentes. Departamento de Computação. Ouro Preto, MG, Brasil.
Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
Max Planck Institute for Molecular Genetics. IBerlin, Germany.
Max Planck Institute for Molecular Genetics. IBerlin, Germany / Freie Universitaet Berlin. Berlin, Germany.
Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brasil.
Abstract: Long noncoding RNAs (lncRNAs) are transcripts generally longer than 200 nucleotides with no or poor protein coding potential, and most of their functions are also poorly characterized. Recently, an increasing number of studies have shown that lncRNAs can be involved in various critical biological processes such as organism development or cancer progression. Little, however, is known about their effects in helminths parasites, such as Schistosoma mansoni. Here, we present a computational pipeline to identify and characterize lncRNAs from RNA-seq data with high confidence from S. mansoni adult worms. Through the utilization of different criteria such as genome localization, exon number, gene length, and stability, we identified 170 new putative lncRNAs. All novel S. mansoni lncRNAs have no conserved synteny including human and mouse. These closest protein coding genes were enriched in 10 significant Gene Ontology terms related to metabolism, transport, and biosynthesis. Fifteen putative lncRNAs showed differential expression, and three displayed sex-specific differential expressions in praziquantel sensitive and resistant adult worm couples. Together, our method can predict a set of novel lncRNAs from the RNA-seq data. Some lncRNAs are shown to be differentially expressed suggesting that those novel lncRNAs can be given high priority in further functional studies focused on praziquantel resistance.
Keywords: Schistosoma mansoni
Cancer progression
keywords: Schistosoma mansoni
Progressão de câncer
Issue Date: 2018
Publisher: BioMed Central
Citation: OLIVEIRA, Victor Fernandes de et al. Identification of 170 new long noncoding RNAs in Schistosoma mansoni. BioMed Research International, v. 2018, p. 1-10, 2018.
DOI: 10.1155/2018/1264697
ISSN: 2314- 6141
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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