Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33007
Title: Evaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis
Authors: Pastor, André Filipe
Rocha, Abraham
Cassemiro, Klécia de Melo
Tenório, Marli
Melo, Paula
Grilis, Maria Rosângela
Rhuama, Maressa
Rezende, Antonio Mauro
Melo Neto, Osvaldo Pompilio de
Marques, Ernesto
Dhalia, Rafael
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil / Instituto Federal de Ciência e Tecnologia do Sertão Pernambucano. Campus Floresta, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil / Hospital Otávio de Freitas. Recife, PE, Brasil.
Instituto Nacional de Câncer. Laboratório de Patologia Clínica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
Abstract: BACKGROUND Lymphatic filariasis (LF) is a parasitic disease caused mainly by the Wuchereria bancrofti worm and that affects up to 120 million people worldwide. LF is the second cause of chronic global deformity, responsible for 15 million people with lymphedema (elephantiasis) and 25 million men with scrotal hydrocele. Its diagnosis is still associated with numerous difficulties, such as the sample collection periods (microfilaria nocturnal periodicity) and limited diagnostic kits. OBJECTIVES The aim of this work was to evaluate two recombinant antigens (Wb14 and WbT) as part of an enzyme-linked immunosorbent assay (ELISA) based antibody capture tests for LF. METHODS The recombinant antigens rWb14 and rWbT were expressed in Escherichia coli BL21 and an antibody capture ELISA was performed. For this, sera were used from microfilaremic individuals with W. bancrofti (MF), chronic pathology (CP), individuals infected with Strongyloides (SP) and healthy controls from endemic (EN) and non-endemic (NE) areas. FINDINGS Both tests showed similar results, with 90% sensitivity and 96.6% specificity. In comparison with the BM14 ELISA commercial test, the Wb14 and WbT antigens performed with identical sensitivity but greater specificity. Reduced positivity with the CP suggested a potential to monitor cure. This was not confirmed, however, when sera from individuals up to seven years after treatment were assayed. MAIN CONCLUSIONS The Wb14 and WbT ELISAs were considered efficient and promising diagnostic tests. Due to the importance of antibody capture analysis to evaluate the Global Program to Eliminate Lymphatic Filariasis (GPELF), the tests proposed here appear as great alternatives to the available commercial system.
Keywords: Immunodiagnostic
Wuchereria bancrofti
ELISA
Biotechnology
DeCS: Animais
Anticorpos, Helmintos / sangue
Antígenos, Helmintos / sangue
Antígenos de Helmintos / imunologia
Estudos de caso-controle
Elefantíase, filarial / diagnóstico
Humanos
Curva ROC
Proteínas Recombinantes / sangue
Proteínas Recombinantes / imunologia
Reprodutibilidade dos resultados
Sensibilidade e Especificidade
Wuchereria bancrofti / imunologia
Issue Date: 2018
Citation: PASTOR, André Filipe et al. Evaluation of the Recombinant Antigens Wb14 and WbT for the Capture Antibody Diagnosis of Lymphatic Filariasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 5, p. 1-8, mar. 2018.
DOI: 10.1590/0074-02760170435
ISSN: 1678-8060
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos




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