| Author | Gomes, Renan Garcia | |
| Author | Brito, Carlos Alexandre Antunes de | |
| Author | Martinelli, Valéria Ferreira | |
| Author | Santos, Rossana Nascimento dos | |
| Author | Gomes, Fabiana Oliveira dos Santos | |
| Author | Peixoto, Christina Alves | |
| Author | Crispim, Janaína Oliveira | |
| Author | Diniz, George Tadeu Nunes | |
| Author | Donadi, Eduardo Antônio | |
| Author | Lucena-Silva, Norma | |
| Access date | 2019-05-09T13:11:46Z | |
| Available date | 2019-05-09T13:11:46Z | |
| Document date | 2018 | |
| Citation | GOMES, R. G. et al. HLA-G Is Expressed in Intestinal Samples of Ulcerative Colitis and Crohn’s Disease Patients and HLA-G5 Expression Is Differentially Correlated with TNF and IL-10 Cytokine Expression. Human Immunology, v. 79, n. 6, p. 477–484, June 2018. | pt_BR |
| ISSN | 1879-1166 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33009 | |
| Sponsorship | CAPES: 1423213 (para RGG) e CNPq: 310364/20159 (para NL-S). | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.humimm.2018.03.006 | |
| Abstract | HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Federal University of Pernambuco. School of Medicine. Department of Internal Medicine. Recife, PE, Brazil. | pt_BR |
| Affilliation | Federal University of Pernambuco. School of Medicine. Department of Internal Medicine. Recife, PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Federal University of Rio Grande do Norte. Department of Pharmacology. Natal, RN, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | University of São Paulo. Medical School of Ribeirão Preto. Department of Internal Medicine. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Subject | Crohn’s disease | pt_BR |
| Subject | HLA-G | pt_BR |
| Subject | IL-10 | pt_BR |
| Subject | Inflammatory bowel disease | pt_BR |
| Subject | TNF | pt_BR |
| Subject | Ulcerative colitis | pt_BR |
| DeCS | Adolescente | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Envelhecido | pt_BR |
| DeCS | Colite Ulcerativa / imunologia | pt_BR |
| DeCS | Doença de Crohn / imunologia | pt_BR |
| DeCS | Progressão da doença | pt_BR |
| DeCS | Células Epiteliais / fisiologia | pt_BR |
| DeCS | Fêmea | pt_BR |
| DeCS | Antígenos HLA-G / metabolismo | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Imunohistoquímica | pt_BR |
| DeCS | Imunomodulação | pt_BR |
| DeCS | Interleucina-10 / genética | pt_BR |
| DeCS | Interleucina-10 / metabolismo | pt_BR |
| DeCS | Mucosa Intestinal / metabolismo | pt_BR |
| DeCS | Intestinos / patologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Meia idade | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | RNA, mensageiro / análise | pt_BR |
| DeCS | Fator alfa de necrose tumoral / genética | pt_BR |
| DeCS | Fator de Necrose Tumoral alfa / metabolismo | pt_BR |
| DeCS | Adulto jovem | pt_BR |
| Embargo date | 2050-01-01 | |
