Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3306
Title: Feto-placentary pathology in human parvovirus B19 infection
Other Titles: Patologia feto-placentária na infecção pelo parvovírus humano B19
Authors: Garcia, Aparecida Gomes Pinto
Pegado, Claudia Schwartz
Cubel, Rita de Cassia
Fonseca, Maria Evangelina Ferreira
Sloboda, Ivan
Nascimento, Jussara Pereira
Affilliation: Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Anatomia Patológica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Virologia. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Departamento de Microscopia Eletrônica. Rio de Janeiro, RJ, Brasil
Abstract: São escassas as referências aos dados histológicos relativos à infecção congênita pelo parvovírus humano B19.Apresentamos estudo morfológico de seis autópsias em que o vírus foi detectado por hibridização DNA (HIS-HDB), PCR e microscopia eletrônica (ME) em tecidos feto-placentários fixados em formol e incluídos em parafina. Estas autópsias integravam um grupo de 86 Hidropisias Fetais não Imunológicas (HFNI) que apresentaram à microscopia óptica complexo lesional sistêmico inflamatório/degenerativo de causa indeterminada. Em uma criança detectou-se processo sifilítico multivisceral com microorganismos, caracterizando infecção dupla. Os fetos exibiram quadro semelhante: hidropisia, hepato-esplenomegalia, hipoplasia pulmonar e eritroblastemia. O dado histológico específico consistiu em inclusão nuclear em eritroblastos do sangue, do tecido visceral eritropoiético e medula óssea. Cardiopatia complexa, lobulação pulmonar anômala e poliesplenia foram observadas em um caso. Em dois corações evidenciou-se fibroelastose difusa. As lesões pulmonares se manifestaram por dismaturidade e processo crônico intersticial; as hepáticas revelaram colestase, fibrose portal, proliferação canalicular, necroses hepatocitárias focais irregularmente dispostas, transformação gigantocitária e hemossiderose. No sistema nervoso central predominaram as lesões anóxicas, embora a autólise não permitisse análise minuciosa.
Abstract: In view of the scarce references concerning the histological data in congenital parvovirus human B19 infection, we intend to provide a description of the pathological features observed in six autopsies.The virus was detected by DNA hybridization (ISH-H),PCR and electronmicroscopy (EM) in paraffin embedded feto-placentary tissues.These cases constitute a subset from 86 Non Immunologic Hydrops Fetalis (NIHF) cases, in which a systemic complex of inflammatory/degenerative lesions of unknown etiology was visualized by optical microscopy. In one case a syphilitic process was detected, typefying a double infection. All fetuses showed a similar pathology - hydrops, hepato-splenomegaly,lung hypoplasia and erythroblastemia, the specific histological feature being the presence of intranuclear inclusions in the erythroid progenitors, in the erythropoietic visceral tissue and in blood marrow. Complex cardiopathy allied to abnormal lung lobulation and polisplenia were observed once; in 2 cases endocardial fibroelastosis was diagnosed. The pulmonary lesions were represented by dysmaturity allied to interstitial mononuclear infiltration. The hepatic consisted of cholestasis, portal fibrosis, canalicular proliferation, hemossiderosis,focal necroses and giant cell transformation. The central nervous system lesions were predominantly anoxic although the autolysis impaired a correct diagnosis.
Keywords: Non immunologic hydrops fetalis
Intrauterin infection
Human parvovirus B19
Morphological study
Virus detection
DeCS: Doenças Placentárias - patologia
Parvovirus B19 Humano - isolamento & purificação
Infecções por Parvoviridae - patologia
Parvovirus B19 Humano - ultraestrutura
Microscopia Eletrônica - métodos
Hibridização Genética
Reação em Cadeia da Polimerase
Autopsia - métodos
Humanos
Recém-Nascido
Issue Date: 1998
Bibliography : 1. ANAND, A.; GRAY, E.S.; BROWEN, T.; CLEWLEY, J.P. & COHEN, B.J. - Human parvovirus infection in pregnancy and hydrops fetalis. New Engl. J. Med., 3l6: 183-186, 1987. [ Links ] 2. ANDERSON, M.J.; KHOUSAM, M. N.; MAXWELL, D.J. et al. - Human parvovirus B19 and hydrops fetalis. Lancet, 1: 535, 1988. [ Links ] 3. BERRY, P.J.; GRAY, E.S.; PORTER, H.J. & BURTON, P.A. - Parvovirus infection of the human fetus and newborn. Semin. Diagn. Path., 9: 4-12, 1992. [ Links ] 4. BOLEY, T.J. & POPEK, E.J. - Parvovirus infection in pregnancy. Semin. Perinat., 17: 410-419, 1993. [ Links ] 5. BURTON, P.A. & CAUL, E.O. - Fetal cell tropism of human parvovirus B19. Lancet, 1:767, 1988. [ Links ] 6. CONRY, J.A.; TOROK, T. & ANDREWS, P.I. - Perinatal encephalopathy secondary to in-utero human parvovirus B19 (HPV) infection. Neurology, 43 (suppl.): A 346, 1993. [ Links ] 7. CUBEL, R.C.N.; GARCIA, A.G.P.; PEGADO, C.S. et al. - Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro. Mem. Inst. Oswaldo Cruz, 9l: 147-151, 1996. [ Links ] 8. ESTERLY, J.R. & OPPENHEIMER, E.H. - Pathological lesions due to congenital rubella. Arch.Path., 87: 380-388, 1969. [ Links ] 9. GARCIA, A.G.P.; PEGADO, C.S.; RAMOS, H.I.B. et al. - Non-immunologic hydrops fetalis. Study of 86 autopsies. Trop. Doctor, 26: 78-79, 1996. [ Links ] 10. GRAY, E.S.; DAVIDSON, R.J. & ANAND, A. - Human parvovirus and fetal anaemia. Lancet, 1: 1144, 1987. [ Links ] 11. MARK, Y.; ROGERS, B.B. & OYER, C.E. - Diagnosis and incidence of fetal parvovirus infection in an autopsy series. II. DNA amplification. Pediat.Path., l3: 381-386, 1993. [ Links ] 12. METZMAN, R.; ANAND, A.; DE GIULIO, P.A. & KNISELY, A.S. - Hepatic disease associated with intrauterine parvovirus B19 infection in a newborn premature infant. J. Pediat. Gastroent. Nutr., 9: 112-114, 1989. [ Links ] 13. MOREY, A.L.; KEELING, J.W.; PORTER, H.J. & FLEMING, K.A. - Clinical and histopathological features of parvovirus B19 infection in the human fetus. Brit. J. Obstet. Gynaec., 99: 566-574, 1992. [ Links ] 14. NEWBOULD, M.J.; ARMSTRONG, G.R. & BARSON, A.J. - Endocardial fibroelastosis in infants with hydrops fetalis. J. clin. Path., 44: 576-579, 199l. [ Links ] 15. PORTER, H.J.; QUANTRILL, A.M. & FLEMING, K. A. - B19 parvovirus infection of myocardial cells. Lancet, l: 535-536, 1988. [ Links ] 16. RODIS, J.F.; HOVICK, T.J.; QUINN, D.L.; ROSENGREN, S.S. & TATTERSALL, P. - Human parvovirus infection in pregnancy. Obstet. & Gynec., 72: 733- 738, 1988. 17. ROGERS, B.B. - Histopathologic variability of finding erythroid inclusions with intrauterine parvovirus B19 infection. Pediat. Path., 12: 883- 889, 1992. [ Links ] 18. USSER, X.T. & DEMMILER, G.J. - Human parvovirus B19. Semin. Pediat. infect. Dis., 7: 89-96, 1996. [ Links ] 19. VAN-ELSACKER-NIELE, A.M.W.; SALIMANS, M.M.M.; WEILAND, H.T. et al. - Fetal pathology in human parvovirus B19 infection. Brit. J. Obstet. Gynaec., 96 : 768-775, 1989. [ Links ] 20. WHITE, F.V.; JORDAN, J.; DICKMAN, P.S.& KNISELY, A.S. - Fetal parvovirus B19 infection and liver disease of antenatal onset in an infant with Ebstein's anomaly. Pediat. Path. Lab. Med., 15: 121-129, 1995. [ Links ]
Citation: GARCIA, Aparecida Gomes Pinto et al. Feto-placentary pathology in human parvovirus B19 infection. Rev. Inst. Med. Trop. São Paulo. São Paulo, v. 40, n. 3, p. 145-150, may/jun. 1998.
DOI: 10.1590/S0036-46651998000300003
ISSN: 0036-4665
Appears in Collections:IFF - Artigos de Periódicos

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