| Author | Guedes, Herbert Leonel de Matos | |
| Author | Carneiro, Monique Pacheco Duarte | |
| Author | Gomes, Daniel Cláudio de Oliveira | |
| Author | Rossi-Bergmann, Bartira | |
| Author | De Simone, Salvatore Giovanni | |
| Access date | 2019-05-16T17:21:34Z | |
| Available date | 2019-05-16T17:21:34Z | |
| Document date | 2007 | |
| Citation | GUEDES, Herbert Leonel de Matos et al. Oligopeptidase B from Leishmania amazonensis: molecular cloning, gene expression analysis and molecular model. Parasitol Res., v. 101, p. 865-875, July 2007. | pt_BR |
| ISSN | 0932-0113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33119 | |
| Description | The name of Dr Bartira Rossi-Bergmann is spelled incorrectly in the
article. Please see the correct spelling above. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania amazonensis | pt_BR |
| Subject in Portuguese | Oligopeptidase B | pt_BR |
| Subject in Portuguese | Clonagem molecular | pt_BR |
| Subject in Portuguese | Análise de expressão gênica | pt_BR |
| Subject in Portuguese | Modelo molecular | pt_BR |
| Title | Oligopeptidase B from Leishmania amazonensis: molecular cloning, gene expression analysis and molecular model | pt_BR |
| Type | Article | |
| Abstract | Serine oligopeptidases of trypanosomatids are
emerging as important virulence factors and therapeutic
targets in trypanosome infections. A complete open reading
frame of oligopeptidase B from Leishmania amazonensis
was amplified with polymerase chain reaction with gradient
annealing temperatures using primers designed for the
oligopeptidase B gene from L. major. The 2,196-bp
fragment coded for a protein of 731 amino acids with a
predicted molecular mass of 83.49 KDa. The encoded
protein (La_OpB) shares a 90% identity with oligopeptidases
of L. major and L. infantum, 84% with L. braziliensis,
and ∼62% identity with Trypanosoma peptidases.
The oligopeptidase B gene is expressed in all cycle stages
of L. amazonensis. The three dimensional model of
La_OpB was obtained by homology modeling based on
the structure of prolyl oligopeptidases. We mapped a
La_OpB model that presents a greater negative charge than
prolyl oligopeptidases; our results suggest a difference in
the S2 subsite when compared to oligopeptidases B from
Trypanosoma and bacterial oligopeptidases B. The La_OpB
model serves as a starting point for its exploration as a
potential target source for a rational chemotherapy. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Sem afiliação. Ver em Notas | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Oligopeptidase B | pt_BR |
| Subject | Molecular cloning | pt_BR |
| Subject | Expression analysis | pt_BR |
| Subject | Modelo molecular | pt_BR |
| e-ISSN | 1432-1955 | |
| Embargo date | 2022-01-01 | |
