Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33119
Title: Oligopeptidase B from Leishmania amazonensis: molecular cloning, gene expression analysis and molecular model
Authors: Guedes, Herbert Leonel de Matos
Carneiro, Monique Pacheco Duarte
Gomes, Daniel Cláudio de Oliveira
Rossi-Bergmann, Bartira
De Simone, Salvatore
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Sem afiliação. Ver em Notas
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Abstract: Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed for the oligopeptidase B gene from L. major. The 2,196-bp fragment coded for a protein of 731 amino acids with a predicted molecular mass of 83.49 KDa. The encoded protein (La_OpB) shares a 90% identity with oligopeptidases of L. major and L. infantum, 84% with L. braziliensis, and ∼62% identity with Trypanosoma peptidases. The oligopeptidase B gene is expressed in all cycle stages of L. amazonensis. The three dimensional model of La_OpB was obtained by homology modeling based on the structure of prolyl oligopeptidases. We mapped a La_OpB model that presents a greater negative charge than prolyl oligopeptidases; our results suggest a difference in the S2 subsite when compared to oligopeptidases B from Trypanosoma and bacterial oligopeptidases B. The La_OpB model serves as a starting point for its exploration as a potential target source for a rational chemotherapy.
Keywords: Leishmania amazonensis
Oligopeptidase B
Molecular cloning
Expression analysis
Modelo molecular
keywords: Leishmania amazonensis
Oligopeptidase B
Clonagem molecular
Análise de expressão gênica
Modelo molecular
Issue Date: 2007
Publisher: Springer Verlag (Germany)
Citation: GUEDES, Herbert Leonel de Matos et al. Oligopeptidase B from Leishmania amazonensis: molecular cloning, gene expression analysis and molecular model. Parasitol Res., v. 101, p. 865-875, July 2007.
Description: The name of Dr Bartira Rossi-Bergmann is spelled incorrectly in the article. Please see the correct spelling above.
ISSN: 0932-0113
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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