| Author | Leitolis, Amanda | |
| Author | Suss, Paula Hansen | |
| Author | Roderjan, João Gabriel | |
| Author | Angulski, Addeli Bez Batti | |
| Author | Costa, Francisco Diniz Affonso da | |
| Author | Stimamiglio, Marco Augusto | |
| Author | Dominguez, Alejandro Correa | |
| Access date | 2019-05-20T14:02:52Z | |
| Available date | 2019-05-20T14:02:52Z | |
| Document date | 2019 | |
| Citation | LEITOLIS, Amanda et al. Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves. Int. J. Mol. Sci. , v. 20, n. 1279, p. 1-18, 2019. | pt_BR |
| ISSN | 1422-0067 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33143 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Explante de Tecido | pt_BR |
| Subject in Portuguese | Recelularização | pt_BR |
| Subject in Portuguese | Regiões Cardíacas | pt_BR |
| Title | Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3390/ijms20061279 | |
| Abstract | Extracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects of EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate human heart-derived extracellular vesicles (hH-EVs). We used nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to physically characterize hH-EVs and mass spectrometry (MS) to profile the protein content in these particles. The MS analysis identified a total of 1248 proteins. Gene ontology (GO) enrichment analysis in hH-EVs revealed the proteins involved in processes, such as the regulation of cell death and response to wounding. The potential of hH-EVs to induce proliferation, adhesion, angiogenesis and wound healing was investigated in vitro. Our findings demonstrate that hH-EVs have the potential to induce proliferation and angiogenesis in endothelial cells, improve wound healing and reduce mesenchymal stem-cell adhesion. Last, we showed that hH-EVs were able to significantly promote mesenchymal stem-cell recellularization of decellularized porcine heart valve leaflets. Altogether our data confirmed that hH-EVs modulate cellular processes, shedding light on the potential of these particles for tissue regeneration and for scaffold recellularization. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Tecnológica Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Subject | Heart | pt_BR |
| Subject | Extracellular Vesicles | pt_BR |
| Subject | Tissue Explant | pt_BR |
| Subject | Heart Valves | pt_BR |
| Subject | Tissue Engineering | pt_BR |
| Subject | Recellularization | pt_BR |
| Subject | Cardiac Regions | pt_BR |
| Subject | Mesenchymal Stromal Cells | pt_BR |
| Subject in Spanish | Corazón | pt_BR |
| Subject in Spanish | Válvulas Cardíacas | pt_BR |
| Subject in Spanish | Ingeniería de Tejidos | pt_BR |
| Subject in Spanish | Células del Estroma Mesenquimatoso | pt_BR |
| DeCS | Coração | pt_BR |
| DeCS | Vesículas Extracelulares | pt_BR |
| DeCS | Valvas Cardíacas | pt_BR |
| DeCS | Engenharia Tecidual | pt_BR |
| DeCS | Células Mesenquimais Estromais | pt_BR |
| e-ISSN | 1422-0067 | |
