Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33143
Title: Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves
Authors: Leitolis, Amanda
Suss, Paula Hansen
Roderjan, João Gabriel
Angulski, Addeli Bez Batti
Costa, Francisco Diniz Affonso da
Stimamiglio, Marco Augusto
Correa, Alejandro
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.
Universidade Tecnológica Federal do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Abstract: Extracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects of EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate human heart-derived extracellular vesicles (hH-EVs). We used nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to physically characterize hH-EVs and mass spectrometry (MS) to profile the protein content in these particles. The MS analysis identified a total of 1248 proteins. Gene ontology (GO) enrichment analysis in hH-EVs revealed the proteins involved in processes, such as the regulation of cell death and response to wounding. The potential of hH-EVs to induce proliferation, adhesion, angiogenesis and wound healing was investigated in vitro. Our findings demonstrate that hH-EVs have the potential to induce proliferation and angiogenesis in endothelial cells, improve wound healing and reduce mesenchymal stem-cell adhesion. Last, we showed that hH-EVs were able to significantly promote mesenchymal stem-cell recellularization of decellularized porcine heart valve leaflets. Altogether our data confirmed that hH-EVs modulate cellular processes, shedding light on the potential of these particles for tissue regeneration and for scaffold recellularization.
Keywords: Heart
Extracellular Vesicles
Tissue Explant
Heart Valves
Tissue Engineering
Recellularization
Cardiac Regions
Mesenchymal Stromal Cells
Keywords in spanish: Corazón
Válvulas Cardíacas
Ingeniería de Tejidos
Células del Estroma Mesenquimatoso
keywords: Explante de Tecido
Recelularização
Regiões Cardíacas
DeCS: Coração
Vesículas Extracelulares
Valvas Cardíacas
Engenharia Tecidual
Células Mesenquimais Estromais
Issue Date: 2019
Publisher: MDPI
Citation: LEITOLIS, Amanda et al. Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves. Int. J. Mol. Sci. , v. 20, n. 1279, p. 1-18, 2019.
DOI: 10.3390/ijms20061279
ISSN: 1422-0067
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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