A list of 135 monogenean species is presented updating the papers published by Kohn & Cohen (1989) and Kohn & Paiva (2000), increasing to 658 the number of known species in South America. After 1997, 104 monogeneans had been described as new species: 65 from Brazil, 13 from Argentina, 10 from Venezuela, 04 from Chile, two from Guyana, one from French Guyana, Peru and Uruguay each. Some species had been originally described in the same publication from more than one country: 04 from Brazil, Peru and Venezuela, two from Brazil and Peru, two from Brazil and Argentina and one from Argentina and Uruguay. More 31 known species had been reported for the first time in South America: 02 from Argentina, 24 from Brazil, one from Colombia and Brazil, one from Chile and Peru, one from Surinam and Brazil and two from Peru. It was also included in the present paper, 62 species which were already listed in Kohn & Cohen (1998) and reported after 1997 in different South American countries. From, Ecuador, Falkland, Galapagos and Paraguay none species had been reported in this period. A host-parasite list is also presented.