Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33173
Title: Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil
Authors: Lima, Manoel Sebastião da Costa
Hartkopf, Andressa Cristina Lopes
Tsujisaki, Rosianne A. de Souza
Oshiro, Elisa Teruya
Shapiro, Julie Teresa
Matos, Maria de Fatima Cepa
Dorval, Maria Elizabeth Cavalheiros
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunopatologia e Biologia Molecular. Recife, PE, Brasil.
Universidade Federal de Mato Grosso do Sul. Laboratory of Molecular Biology and Cell Culture. Campo Grande, MS, Brazil.
Universidade Federal de Mato Grosso do Sul. Laboratory of Molecular Biology and Cell Culture. Campo Grande, MS, Brazil.
Universidade Federal de Mato Grosso do Sul. Center for Biological and Health Sciences. Campo Grande, MS, Brazil.
University of Florida. School of Natural Resources and the Environment. Department of Wildlife Ecology and Conservation. Gainesville, FL, USA.
Universidade Federal de Mato Grosso do Sul. Laboratory of Molecular Biology and Cell Culture. Campo Grande, MS, Brazil.
Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Graduate Program in Infectious and Parasitic Diseases. Campo Grande, MS, Brazil.
Abstract: Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient. These results suggest the feasibility of using urine samples to diagnose visceral leishmaniasis, especially in acute cases or renal infection, providing a valuable tool for doctors and clinicians to use for screening and diagnosis of leishmaniasis in patients.
Keywords: Diagnosis
Leishmania
PCR
Urine
Zoonosis
DeCS: Animais
Brasil
Humanos
Leishmania infantum / isolamento & purificação
Leishmaniose Visceral / diagnóstico
Leishmaniose Visceral / parasitologia
Leishmaniose Visceral / urina
Reação em Cadeia da Polimerase / métodos
Issue Date: 2018
Citation: DA COSTA LIMA, M. S. et al. Isolation and Molecular Characterization of Leishmania Infantum in Urine from Patients with Visceral Leishmaniasis in Brazil. Acta Tropica, v. 178, p. 248–251, fev. 2018.
DOI: 10.1016/j.actatropica.2017.12.011
ISSN: 1873-6254
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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