Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33186
Title: Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot
Authors: Cruz, Helena Medina
Cruz, Juliana Custódio Miguel
Silva, Elisangela Ferreira da
Portilho, Moyra Machado
Marques, Vanessa Alves
Lewis-Ximenez, Lia Laura
Lampe, Elisabeth
Villar, Livia Melo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Abstract: Dried blood spots (DBSs) could be an alternative to serum for hepatitis B virus (HBV) and hepatitis C virus (HCV) diagnosis. This study aims to evaluate two enzyme immunoassays (EIAs) for HBsAg and anti-HCV detection using DBS. Serum was tested using commercial EIA. DBS was tested using optimized EIA developed for serum and commercial EIA developed for DBS (Imunoscreen). Concordances between DBS and serum samples for both markers and EIAs were higher than 97%. Both EIAs demonstrated good performance for HBsAg and anti-HCV detection using DBS, and these methods could be used unchangeably increasing the access for HBV and HCV diagnosis.
Keywords: Dried blood spots
Hepatitis B
Hepatitis C
Enzyme immunoassays
keywords: Manchas de sangue seco
Hepatite B
Hepatite C
Imunoensaios enzimáticos
Issue Date: 2018
Publisher: Taylor & Francis
Citation: CRUZ, Helena Medina et al. Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot. Journal of Immunoassay and Immunochemistry, v. 39, n. 2, p. 228-233, 2018.
DOI: 10.1080/15321819.2018.1470095
ISSN: 1532-1819
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
ElizabethLampe_LiviaVillar_etal_IOC_2018.pdf1.58 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.