Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33213
Title: In vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection
Authors: Aranda-Souza, Mary Ângela
Lorena, Virginia Maria Barros de
Correia, Maria Tereza dos Santos
Figueiredo, Regina Célia Bressan Queiroz de
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Departamento de Bioquímica Laboratório de Proteínas. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Abstract: Leishmania amazonensis and Leishmania braziliensis are the main causative agents of American Tegumentary Leishmaniasis (ATL) in Brazil. As intracellular parasites, the infection by Leishmania species is dependent on the host immune response and the immunotherapy could be promissory for the development of new strategies to combat ATL. In this work we investigated the leishmanicidal potential of a galactose-binding lectin from the snake venom of Bothrops leucurus (BLL) during the infection with L. amazonensis and L. braziliensis. BLL inhibited the promastigote growth and viability of both species in a mechanism dependent on galactose and calcium. The treatment with BLL also decreases the survival of intracellular parasites for both species and induced profound ultrastructural changes on amastigotes without apparent damage to the host cells. The analysis of the cytokine profile revealed that BLL induced an increase in the proinflammatory cytokines IL-6 and TNF-α by infected macrophages in both species, but differed in relation to IL-1β and IL-10 response. Future works using in vitro and in vivo models are necessary to support the use of these lectins as biotechnological tool in immunological studies.
Keywords: Lectin
Leishmania
Immunomodulation
DeCS: Animais
Bothrops
Brasil
Galectinas / química
Galectinas / farmacologia
Humanos
Leishmania braziliensis / efeitos de drogas
Leishmania braziliensis / patogenicidade
Leishmaniose cutânea / quimioterapia
Leishmaniose cutânea / parasitologia
Macrófagos / efeitos de drogas
Ratos
Venenos de cobra / química
Venenos de cobra / farmacologia
Issue Date: 2018
Citation: ARANDA-SOUZA, Mary Ângela et al. In vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection. International Journal of Biological Macromolecules, v. 120, p. 431-439, Dec. 2018.
DOI: 10.1016/j.ijbiomac.2018.08.064
ISSN: 1879-0003
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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