Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33448
Title: Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
Authors: Teixera, Leda Fabiélen
Carvalho, Letícia Helena de
Castro, Onássis Boeri de
Bastos, Jéssica Silva Félix
Néry, Neriane Monteiro
Oliveira, George Azevedo
Kayano, Anderson Makoto
Soares, Andreimar Martins
Zuliani, Juliana Pavan
Affilliation: Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Centro Universitário São Lucas. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Abstract: The present work aimed to isolate a basic phospholipase A2 (PLA2) from Bothrops diporus snake venom (BdV), evaluate and compare the myotoxic and oedema-inducing activities, as well as the systemic effects caused by both the isolated PLA2 and BdV on Swiss mice. A Lys-49 PLA2 (BdipTX-I) was obtained through two chromatographic steps: an ion-exchange and a reverse phase. The local (oedema and myotoxicity) and systemic (hepatic and renal functions) effects were then assessed for BdipTX-I and BdV. Results showed that the oedema-inducing activity was significant in all tested doses (5 and 20 mg/paw) for both BdipTX-I and BdV. Myotoxicity was evaluated by the increase of serum CK, CK-MB and LDH, and results showed that BdV effect is more prominent than BdipTX-I effect. The systemic effects were evaluated by determining specific laboratory markers: AST, ALT, GGT, ALP, urea, creatinine, protein and calcium. BdipTX-I and BdV were able to induce renal changes in the experimental model, leading to proteinuria (induced both by BdipTX-I and by BdV) and uremia (induced only by BdV). Thus, it is concluded that the systemic effects of BdV and BdipTX-I occur differently.
Keywords: Phospholipase A2
Bothrops diporus
Oedema
Myotoxicity
Systemic effect
Issue Date: 2017
Publisher: Elsevier
Citation: TEIXEIRA, Leda Fabiélen et al. Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom. Toxicon, v. 141, p. 55-64, 2017.
Description: O artigo encontra-se disponível para download no site do Editor.
DOI: 10.1016/j.toxicon.2017.11.007
ISSN: 0041-0101
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos




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