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2020-06-13
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A COMPARATIVE SEROLOGICAL STUDY OF THE SSCBF ANTIGENIC FRACTION ISOLATED FROM THREE SPOROTHRIX SCHENCKII STRAINS
Bernardes-Engemann, A. R. et al. | Date Issued: 2009
Author
Bernardes-Engemann, A. R.
Loureiro y Penha, C. V.
Benvenuto, F.
Braga, J. U.
Barros, M. B. L.
Orofino Costa, R.
Lopes-Bezerra, L.
Affilliation
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Pós-Graduação em Fisiopatologia Clínica e Experimental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.
Abstract
A previous work showed that an antigenic fraction of Sporothrix schenckii , Ss CBF, was specifi cally recognized by IgG antibodies present in patients’ clinical specimens. The objective of this investigation was to compare the reactivity of this antigen recovered from three S. schenckii strains. ROC curve analysis revealed a variation in the sensitivity and specifi city of the antigen derived from each of the strains, with a higher AUC for strain 1099-18. Moreover, the presence of the main O-glycosidically-linked epitopes described in the Ss CBF fraction was ascertained. A signifi cant reduction in Ss CBF reactivity of all the strains was observed after β-elimination confi rming the presence of O-glycan epitopes. The antigen isolated from strain 1099-18 proved to be a more accurate diagnostic tool for serodiagnosis of sporotrichosis.
Keywords
Sporotrichosis
Serology
Sporothrix schenckii
SsCBF antigen
ROC analysis
 
Publisher
Oxford University Press
Citation
BERNARDES-ENGERMANN, Andréa R. et al. A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains. Medical Mycology, v. 47, p. 874-878, Dec. 2009.
DOI
10.3109/13693780802695520
ISSN
1369-3786