Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33540
Title: Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits
Authors: Affonso, Filipe de Souza
Cailleaux, Solange
Pinto, Leonardo Felipe Corrêa
Gomes, Marilia de Brito
Tibiriçá, Eduardo
Affilliation: Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Farmacologia Neuro Cardiovascular. Rio de Janeiro, RJ. Brasil.
Abstract: Objective – To assess the acute effects of high glucose concentrations on vascular reactivity in the isolated non diabetic rabbit kidney. Methods – Rabbits were anaesthetized for isolation of the kidneys. Renal arteries and veins were cannulated for perfusion with Krebs-Henselleit solution and measurement of perfusion pressure. After 3 hours of perfusion with glucose 5,5 mM (control ) and 15 mM, the circulation was submitted to sub maximal precontraction (80% of maximal response) trough continuous infusion of noradrenaline 10 mM. Vascular reactivity was then assessed trough dose–responses curves with endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilators. The influence of hyperosmolarity was analyzed with perfusion with mannitol 15mM. Results – A significant reduction in the endotheliumdependent vasodilation in glucose 15mM group was observed compared to that in control, but there was no difference in endothelium-independent vasodilation. After perfusion with mannitol 15 mM, a less expressive reduction in endothelium-dependent vasodilation was observed, only reaching significance in regard to the greatest dose of acetylcholine. Conclusions – High levels of glucose similar to those found in diabetic patients in the postprandial period can cause significant acute changes in renal vascular reactivity rabbits. In diabetic patients these effects may also occur and contribute to diabetes vascular disease.
Keywords: Endothelial dysfunction
Hyperglycemia
Renal circulation
keywords: Disfunção endotelial
Hiperglicemia
Circulação Renal
Issue Date: 2003
Publisher: Sociedade Brasileira de Cardiologia
Citation: AFFONSO, Filipe de Souza et al. Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits. Arquivos Brasileiros de Cardiologia, v. 81, n. 1, p. 161-165, 2003.
DOI: 10.1590/S0066-782X2003001000005
ISSN: 0066-782X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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