First parasitological, histopathological and molecular characterization of Echinococcus vogeli Rausch and Bernstein, 1972 from Cuniculus paca Linnaeus, 1766 in the Cerrado biome (Mato Grosso do Sul, Brazil)

Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Acre Federal Institute. Xapuri, AC, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço de Zoonoses. Rio de Janeiro, RJ, Brasil.
State University of Rio de Janeiro. Faculty of Medical Sciences. Biomedical Centre. Department of Pathology. Rio de Janeiro, RJ, Brazil / Estácio de Sá University. School of Medicine. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
State University of Rio de Janeiro. Faculty of Medical Sciences. Biomedical Centre. Department of Microbiology, Immunology and Parasitology. Romero Lascasas Porto Laboratory of Helminthology. Rio de Janeiro, RJ, Brazil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.

Abstract

Polycystic echinococcosis (PE) is caused by Echinococcus vogeli metacestodes (larval stage) in Neotropical countries. E. vogeli is trophically-transmitted between predators bush dogs (Speothos venaticus) and prey pacas (Cuniculus paca). In Brazil, reported PE cases are restricted to the Amazon biome. In this study, metacestodes from a paca hunted in Mato Grosso do Sul state (Cerrado biome) were identified morphological and histopathological techniques and further confirmed by molecular testing (sequencing of cytochrome C oxidase subunit I (cox1) gene) for the first time. Images of the whole liver showed superficial bubble-like hepatic masses. The parasitological analysis revealed large hooks (41.3 ± 1.2 μm length/12.8 ± 0.8 μm width) and small hooks (33.0 ± 1.5 μm length/11.1 ± 1.2 μm width), consistent with E. vogeli. Microscopically, the liver showed protoscoleces, a thick laminated layer, fibrosis, and inflammatory infiltrate in the adventitial layer. The DNA sequencing confirmed E. vogeli with 99% homology with sequences deposited in the GenBank. In addition, this finding greatly extends the geographic range of animal polycystic echinococcosis into the Cerrado. It is likely to occur in new biomes, where bush dogs and pacas share a given area in a trophic relationship.

Keywords

Echinococcus vogeli
Cuniculus paca
Liver lesion
Parasitological diagnosis
Histopathological diagnosis
Molecular characterization

Publisher

Elsevier

Citation

BITTENCOURT-OLIVEIRA, Fernanda et al. First parasitological, histopathological and molecular characterization of Echinococcus vogeli Rausch and Bernstein, 1972 from Cuniculus paca Linnaeus, 1766 in the Cerrado biome (Mato Grosso do Sul, Brazil). Veterinary Parasitology, v. 250, p. 35-39, 2018.

DOI

10.1016/j.vetpar.2017.12.003

ISSN

0304-4017

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33750

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