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MODIFICATION AND OPTIMIZATION OF THE FECPAKG2 PROTOCOL FOR THE DETECTION AND QUANTIFICATION OF SOIL-TRANSMITTED HELMINTH EGGS IN HUMAN STOOL
Ayana, Mio et al. | Date Issued: 2018
Author
Ayana, Mio
Vlaminck, Johnny
Cools, Piet
Ame, Shaali
Albonico, Marco
Dana, Daniel
Keiser, Jennifer
Manly, Helen
Matoso, Leonardo Ferreira
Mekonnen, Zeleke
Montresor, Antonio
Oliveira, Rodrigo Corrêa de
Rinaldi, Laura
Sayasone, Somphou
Sowersby, Stephen
Tesfaye, Lensa
Vercruysse, Jozef
Mirams, Greg
Levecke, Bruno
Affilliation
Institute of Health. Faculty of Health Science. School of Medical Laboratory Science. Jimma University. Jimma, Ethiopia.
Department of Virology, Parasitology and Immunology. Ghent University. Merelbeke, Belgium.
Institute of Health. Faculty of Health Science. School of Medical Laboratory Science. Jimma University. Jimma, Ethiopia.
Public Health Laboratory-Ivo de Carneri. Chake Chake, United Republic of Tanzania.
Center for Tropical Diseases. Sacro Cuore Don Calabria Hospital. Negrar, Italy/Department of Life Sciences and Systems Biology. University of Turin. Turin, Italy.
Institute of Health. Faculty of Health Science. School of Medical Laboratory Science. Jimma University. Jimma, Ethiopia.
Swiss Tropical and Public Health Institute. Basel, Switzerland.
Techion Group Ltd. Dunedin, New Zealand.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Institute of Health. Faculty of Health Science. School of Medical Laboratory Science. Jimma University. Jimma, Ethiopia.
World Health Organization. Geneva, Switzerland.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil.
Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy.
National Institute of Public Health.Ministry of Health. Vientiane, Lao People's Democratic Republic.
Department of Biochemistry. University of Otago. Otago, New Zealand.
Institute of Health. Faculty of Health Science. School of Medical Laboratory Science. Jimma University. Jimma, Ethiopia.
Department of Virology, Parasitology and Immunology. Ghent University. Merelbeke, Belgium.
Techion Group Ltd. Dunedin, New Zealand.
Department of Virology, Parasitology and Immunology. Ghent University. Merelbeke, Belgium.
Abstract
Today, infections with soil-transmitted helminths are diagnosed by looking for the eggs of these helminths in stool samples using different microscopic techniques. However, these standard diagnostic techniques still show a number of limitations. The FECPAKG2 method was recently developed for diagnosing worm infections in the veterinary field and could also be useful to diagnose human helminth infections. This method produces digital images of helminth eggs that have been concentrated into one microscopic field of view and stores these images online. This eliminates the need for skilled technicians and opens the possibility for standardized quality control and possibly even automated egg counting by egg recognition software. This study presents, for the first time, a standard operating FECPAKG2 procedure for the detection and quantification of human STH eggs. We optimized the sedimentation and accumulation steps in the FECPAKG2 procedure for the detection of human helminth eggs using stool samples from naturally infected children. This established protocol allows for a thorough and objective evaluation of the system as a diagnostic tool that shows potential for implementation in future STH control programs.
Keywords in Portuguese
Sedimentação
Helmintíases transmitidas pelo solo
Ancilostomídeos
Trichuris
helmintos
Ascaris
Infecções helmínticas
 
Keywords
Sedimentation
Soil-transmitted helminthiases
Hookworms
Trichuris
Helminths
Slurries
Ascaris
Helminth infections
 
Publisher
Public Library of Science
Citation
AYANA, Mio et al. Modification and optimization of the FECPAKG2 protocol for the detection and quantification of soil-transmitted helminth eggs in human stool. PLoS Neglected Tropical Diseases, v. 12, n. 10, p. 1-16, 2018.
DOI
10.1371/journal.pntd.0006655
ISSN
1935-2735