Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33898
Title: Impaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect
Authors: Almeida, Vinícius Cotta de
Villa-Verde, Déa Maria Serra
Lepault, Françoise
Pléau, Jean-Marie
Dardenne, Mireille
Savino, Wilson
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Hôpital Necker. CNRS UMR-8147. Paris, France.
Hôpital Necker. CNRS UMR-8147. Paris, France.
Hôpital Necker. CNRS UMR-8147. Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Hôpital Necker. CNRS UMR-8147. Paris, France.
Abstract: We previously showed intrathymic alterations in non-obese diabetic (NOD) mice, including the appearance of giant perivascular spaces, filled with mature thymocytes, intermingled with an extracellular matrix network. This raised the hypothesis of a defect in thymocyte migration with partial arrest of exiting thymocytes in the perivascular spaces. Herein, we investigated the expression of receptors for fibronectin [very late antigen (VLA)-4 and VLA-5] and laminin (VLA-6), known to play a role in thymocyte migration. When compared with two normal and one other autoimmune mouse strains, a decrease of VLA-5 expression in NOD thymocytes was noticed, being firstly observed in late CD4/CD8 double-negative cells, and more pronounced in mature CD4(+) and CD8(+) thymocytes. Functionally, thymocyte exit from the lymphoepithelial complexes, the thymic nurse cells, was reduced. Moreover, NOD thymocyte adhesion to thymic epithelial cells as well as to fibronectin was diminished, and so was the migration of NOD thymocytes through fibronectin-containing transwell chambers. In situ, intra-perivascular space thymocytes were VLA-5-negative, suggesting a correlation between the thymocyte arrest within these structures and loss of VLA-5 expression. Overall, our data reveal impairment in NOD thymocyte migration, and correspond to the first demonstration of a functional fibronectin receptor defect in the immune system.
Keywords: NOD mice
Thymocyte migration
VLA
Integrins
Fibronectin
keywords: Migração de timócitos
Fibronectina
Integrinas
DeCS: Receptores de Antígeno muito Tardio
Camundongos Endogâmicos NOD
Issue Date: 2004
Publisher: Wiley 12 Months
Citation: ALMEIDA, Viniicius Cotta de et al. Impaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect. Eur. J. Immunol., v. 34, p. 1578-1587, 2004.
DOI: 10.1002/eji.200324765
ISSN: 0014-2980
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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