Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/34782
Title: The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis
Authors: Melo, Tatiane Teixeira de
Mendes, Mariana Moreira
Alves, Clarice Carvalho
Carvalho, Gardênia Braz
Fernandes, Viviane Cristina
Pimenta, Deborah Laranjeira Ferreira
Mourão, Marina de Moraes
Gai, Fatou
Kalli, Marina
Coelho, Aline
Ribeiro, Rosy Iara Maciel de Azambuja
Falcone, Franco H.
Pereira, Rosiane Aparecida da Silva
Fonseca, Cristina Toscano
Affilliation: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.
The School of Pharmacy. University of Nottingham. Nottingham, United Kingdom.
The School of Pharmacy. University of Nottingham. Nottingham, United Kingdom.
Universidade Federal de São João Del Rei. Laboratório de Patologia Experimental. MG, Brasil.
Universidade Federal de São João Del Rei. Laboratório de Patologia Experimental. MG, Brasil.
The School of Pharmacy. University of Nottingham. Nottingham, United Kingdom
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Abstract: Great efforts have been made to identify promising antigens and vaccine formulations against schistosomiasis. Among the previously described Schistosoma vaccine candidates, cyclophilins comprise an interesting antigen that could be used for vaccine formulations. Cyclophilin A is the target for the cyclosporine A, a drug with schistosomicide activity, and its orthologue from Schistosoma japonicum induces a protective immune response in mice. Although Schistosoma mansoni cyclophilin A also represents a promising target for anti-schistosome vaccines, its potential to induce protection has not been evaluated. In this study, we characterized the cyclophilin A (SmCyp), initially described as Smp17.7, analyzed its allergenic potential using in vitro functional assays, and evaluated its ability to induce protection in mice when administered as an antigen using different vaccine formulations and strategies. Results indicated that SmCyp could be successfully expressed by mammalian cells and bacteria. The recombinant protein did not promote IgE-reporter system activation in vitro, demonstrating its probable safety for use in vaccine formulations. T and B-cell epitopes were predicted in the SmCyp sequence, with two of them located within the active isomerase site. The most immunogenic antigen, SmCyp (107–121), was then used for immunization protocols. Immunization with the SmCyp gene or protein failed to reduce parasite burden but induced an immune response that modulated the granuloma area. In contrast, immunization with the synthetic peptide SmCyp (107–121) significantly reduced worm burden (48–50%) in comparison to control group, but did not regulate liver pathology. Moreover, the protection observed in mice immunized with the synthetic peptide was associated with the significant production of antibodies against the SmCyp (107–121) epitope. Therefore, in this study, we identified an epitope within the SmCyp sequence that induces a protective immune response against the parasite, thus representing a promising antigen that could be used for vaccine formulation against schistosomiasis.
Keywords: Cyclophilin A
Epitope
Immunization
Schistosoma mansoni
Issue Date: 2019
Publisher: Pergamon Press
Citation: MELO, Tatiane Teixeira de et al. The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis. Molecular Immunology, v. 111, p. 172-181, 2019.
DOI: 10.1016/j.molimm.2019.04.021
ISSN: 0161-5890
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos




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