Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/34869
Title: Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
Authors: Iñiguez, Alena Mayo
Araújo, Adauto José Goncalves de
Silva, Luiz Fernando Rocha Ferreira da
Vicente, Ana Carolina Paulo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.
Abstract: The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.
Keywords: Molecular Paleoparasitology
Coprolites
Random Amplified Polymorphic DNA
Ancient DNA
Polymerase Chain Reaction
Issue Date: 2003
Publisher: Instituto Oswaldo Cruz
Citation: IÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003.
DOI: 10.1590/S0074-02762003000900012
ISSN: 0074-0276
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos

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