Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Ciêencias e Tecnologias Agropecúarias. Laboratório de Mehoramento e Genética Vegetal. Campos de Goytacazes, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Química e Função de Proteínas e Pept[ideos. Campos de Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Química e Função de Proteínas e Pept[ideos. Campos de Goytacazes, RJ, Brasil.
Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.

Abstract

There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml-1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi 'in vitro' There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

Keywords in Portuguese

Colletotrichum gloeosporioides
Análise bioquímica
Peptídeos antimicrobianos
Genótipos de capsicum
Infecção de frutas

Keywords

Colletotrichum gloeosporioides
Biochemical analysis
Antimicrobial peptides
Capsicum genotypes
Fruit infection

Publisher

Portland Press

Citation

MARACAHIPES, Álan C. et al. Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides. Bioscience Reports, v. 39, p. 1-14, 2019.

DOI

10.1042/BSR20181889

ISSN

0144-8463

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35038

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Open access

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