Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35049
Title: Blackflies in the ointment: O. volvulus vector biting can be significantly reduced by the skin-application of mineral oil during human landing catches
Authors: Silva, Túllio Romão Ribeiro da
Crainey, James Lee
Pessoa, Felipe Arley Costa
Santos, Yago Vinícius Serra dos
Silva, Jordam William Pereira
Leles, Lorena Ferreira de Oliveira
Vicente, Ana Carolina
Luz, Sérgio Luiz Bessa
Affilliation: Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação Stricto sensu em Biologia Parasitária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil / Programa de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro (PPGBIO-Interação), Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil / Programa de Pós-Graduação Stricto Sensu em Condições de Vida e Situações de Saúde na Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil / Programa de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro (PPGBIO-Interação), Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil..
Programa de Pós-Graduação Stricto Sensu em Condições de Vida e Situações de Saúde na Amazônia, Manaus, Amazonas, Brasil.
Abstract: Standard human landing catches (sHLCs) have historically been a key component of Onchocerca volvulus transmission monitoring, but expose health-workers to potentially hazardous vector bites. Novel human-bait-free trapping methods have been developed, but do not always work where they are needed and may not generate O. volvulus surveillance data that is directly comparable with historic data. Methodology Simuliid sHLCs and mineral-oil protected HLCs (mopHLCs) were performed in a rural village of Amazonas state, Brazil. A four-hour direct comparisons of sHLCs and mopHLCs was carried-out using six vector collectors, each of whom used one leg for a sHLC and one for a mopHLC. Two-person collection teams then exclusively performed either mopHLCs or sHLCs for a further set of 12 four-hour collections. Following the completion of all collections, simuliid-bite mark estimates were made from legs used exclusively in sHLCs and legs used exclusively in mopHLCs. Principal findings All of the 1669 captured simuliids were identified as the O. volvulus vector Simulium oyapockense. Overall, mopHLC simuliids captured per hour (S/H) rates were lower than those obtained with sHLC trapping (15.5 S/H versus 20 S/H). Direct comparisons of simuliid capture rates found that vector-collectors captured simuliids significantly more efficiently (x 20.5 S/H) with mopHLC trapping than with sHLC trapping (x�: 16.4 S/H): P-value = 0.002. MopHLCs performed in isolation were, however, observed to capture vectors less efficiently (x�: 13.4 S/H) than sHLCs performed under similar conditions (x�: 19.98 S/H). All six vector collectors had significantly higher simuliid capture per counted bite mark (SC/CBM) rates using mopHLCs than they were observe to have using sHLCs (x�: 21 SC/CBM versus x�: 1 SC/CBM; p-value = 0.03125). Conclusions Vector collectors captured significantly more simuliids per counted bite mark with mopHLCs than with sHLCs. Further investigations into the utility of mopHLCs for onchocerciasis xenomonitoring and beyond are merited.
Keywords: Black flies
O. volvulus vector biting
Ointment
Skinapplication of mineral oil
Humans
keywords: Moscas negras
Pomada
O. volvulus vetor
Aplicação de óleo mineral
Redução
Picadas em Humanos
Issue Date: 2019
Publisher: Public Library of Science
Citation: SILVA, Túllio Romão Ribeiro da et al. Blackflies in the ointment: O. volvulus vector biting can be significantly reduced by the skinapplication of mineral oil during human landing catches. PLoS Neglected Tropical Disease, v. 13, n. 4, p. 1-19, April 2019.
DOI: 10.1371/journal.pntd.0007234
ISSN: 1935-2727
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos
IOC - Artigos de Periódicos

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