Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35064
Title: Anti-platelet aggregation activity of two novel acidic Asp49-phospholipases A2 from Bothrops brazili snake venom
Authors: Sobrinho, Juliana C.
Kayano, Anderson M.
Silva, Rodrigo Simões
Alfonso, Jorge J.
Gomez, Ana F.
Gomez, Maria C. Vega
Zanchi, Fernando B.
Moura, Laura A.
Souza, Vivian R.
Fuly, André L.
Oliveira, Eliandre de
Silva, Saulo L. da
Almeida, José R.
Zuliani, Juliana P.
Soares, Andreimar M.
Affilliation: Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Centro para el Desarrollo de la Investigación Científica. Asunción, Paraguay.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Centro para el Desarrollo de la Investigación Científica. Asunción, Paraguay.
Centro para el Desarrollo de la Investigación Científica. Asunción, Paraguay.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Instituto de Biologia. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Instituto de Biologia. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Instituto de Biologia. Niterói, RJ, Brasil.
Barcelona Science Park. Proteomics Platform. Barcelona, Spain.
Universidade Regional Amazónica. Napo, Ecuador.
Universidade Regional Amazónica. Napo, Ecuador.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Centro Universitário São Lucas. Porto Velho, RO, Brasil.
Abstract: Phospholipases A2(PLA2s) are important enzymes present in snake venoms and are related to a wide spec-trum of pharmacological effects, however the toxic potential and therapeutic effects of acidic isoformshave not been fully explored and understood. Due to this, the present study describes the isolation andbiochemical characterization of two new acidic Asp49-PLA2s from Bothrops brazili snake venom, namedBraziliase-I and Braziliase-II. The venom was fractionated in three chromatographic steps: ion exchange,hydrophobic interaction and reversed phase. The isoelectric point (pI) of the isolated PLA2s was deter-mined by two-dimensional electrophoresis, and 5.2 and 5.3 pIs for Braziliase-I and II were observed,respectively. The molecular mass was determined with values of 13,894 and 13,869 Da for Braziliase-I and II, respectively. Amino acid sequence by Edman degradation and mass spectrometry completed87% and 74% of the sequences, respectively for Braziliase-I and II. Molecular modeling of isolated PLA2susing acid PLA2BthA-I-PLA2from B. jararacussu template showed high quality. Both acidic PLA2s showedno significant myotoxic activity, however they induced significant oedematogenic activity. Braziliase-Iand II (100 g/mL) showed 31.5% and 33.2% of cytotoxicity on Trypanosoma cruzi and 26.2% and 19.2%on Leishmania infantum, respectively. Braziliase-I and II (10 g) inhibited 96.98% and 87.98% of plateletaggregation induced by ADP and 66.94% and 49% induced by collagen, respectively. The acidic PLA2s bio-chemical and structural characterization can lead to a better understanding of its pharmacological effectsand functional roles in snakebites pathophysiology, as well as its possible biotechnological applicationsas research probes and drug leads.
Keywords: Bothrops brazili
Snake venom
Acidic phospholipases A2
Anti-platelet aggregation activity
Issue Date: 2018
Publisher: International Journal of Biological Macromolecules
Citation: SOBRINHO, Juliana C. et al. Anti-platelet aggregation activity of two novel acidic Asp49-phospholipases A2 from Bothrops brazili snake venom. International Journal of Biological Macromolecules, v. 107, p. 1014-1022, 2018.
DOI: 10.1016/j.ijbiomac.2017.09.069
ISSN: 0141-8130
Copyright: restricted access
Appears in Collections:RO - Artigos de Periódicos




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