Author | Caragata, Eric Pearce | |
Author | Rocha, Marcele Neves | |
Author | Pereira, Thiago Nunes | |
Author | Mansur, Simone Brutman | |
Author | Dutra, Heverton Leandro Carneiro | |
Author | Moreira, Luciano Andrade | |
Access date | 2019-08-26T13:44:59Z | |
Available date | 2019-08-26T13:44:59Z | |
Document date | 2019 | |
Citation | CARAGATA, Eric Pearce et al. Pathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-26, 2019. | pt_BR |
ISSN | 1935-2735 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/35065 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Pathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0007443 | |
Abstract | Background: Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment.
Methodology/principle findings: Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner.
Conclusions/significance: Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Mosquitoes | pt_BR |
Subject | Co-infections | pt_BR |
Subject | Wolbachia | pt_BR |
Subject | Arboviral infections | pt_BR |
Subject | Saliva | pt_BR |
Subject | Viral pathogens | pt_BR |
Subject | Viral transmission and infection | pt_BR |
Subject | Viral load | pt_BR |