Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35092
Title: Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
Authors: Santos, Isabele Barbieri dos
Tortelly, Rogerio
Quintella, Leonardo Pereira
Madeira, Maria de Fátima
Miranda, Luisa Helena Monteiro de
Figueiredo, Fabiano Borges
Oliveira, Raquel de Vasconcellos Carvalhaes de
Schubach, Tânia Maria Pacheco
Affilliation: Fundação Oswaldo Cruz. DIREB. Programa de Epidemiologia e Vigilância em Saúde. Brasília, DF, Brasil.
Universidade Federal Fluminense. Faculdade de Veterinária. Serviço de Anatomia Patológica Doutor Jefferson Andrade dos Santos. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Parasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Epidemiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.
Abstract: Background: The in situ detection of parasite antigens in tissue sections by immunohistochemistry (IHC) is a diagnostic alternative for human American tegumentary leishmaniasis (ATL), but has not been used for the diagnosis of cutaneous lesions in dogs with ATL. This study describes the results of IHC for the detection of amastigote forms and other Leishmania sp. antigen-positive cells and compares the results of IHC, histopathology and cytopathology for the diagnosis of canine ATL. In addition, possible cross-reactivity with sporotrichosis is analyzed. Methods: Forty paraffin-embedded biopsies and 40 smears of cutaneous lesions from dogs with ATL, confirmed by isolation and characterization of Leishmania (Viannia) braziliensis, and 40 paraffin-embedded biopsies of cutaneous lesions from dogs with sporotrichosis, confirmed by isolation of Sporothrix schenckii in culture (control group), were studied. Results: Immunohistochemistry was more sensitive in detecting amastigote forms than cytopathology and histopathology, with a positivity rate of 70% (n¼28) versus 37.5% and 22.5% for histopathology and cytopathology, respectively. Cytoplasmic staining of mononuclear and endothelial cells was detected by IHC, which was highly specific since no cytoplasmic staining of these cells or staining of fungal structures was observed in sporotrichosis fragments. Conclusions: In view of the higher sensitivity of IHC in detecting Leishmania sp. antigen and patterns of positivity for Leishmania sp. antigen compared to histopathology or cytopathology and the absence of cross-reactions with sporotrichosis, we recommend this technique for the diagnosis of canine tegumentary leishmaniasis.
Keywords: American tegumentary leishmaniasis
Brazil
Dogs
Immunohistochemistry
Issue Date: 2015
Publisher: Oxford University Press
Citation: SANTOS, Isabele Barbieri dos et al. Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 109, p. 469-476, 2015.
DOI: 10.1093/trstmh/trv034
ISSN: 0035-9203
Copyright: restricted access
Appears in Collections:BSB - Artigos de Periódicos
INI - Artigos de Periódicos

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