Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35589
Title: Genetic and biological characterisation of Zika virus isolates from different brazilian regions
Authors: Strottmann, Daisy Maria
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Koishi, Andrea Cristine
Auwerter, Nathalia Cavalheiro
Faoro, Helisson
Cataneo, Allan Henrique Depieri
Kuczera, Diogo
Wowk, Pryscilla Fanini
Bordignon, Juliano
Santos, Claudia Nunes Duarte dos
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Abstract: Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 brazilian epidemics. As methods for this study, the ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. As findings, are eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the ellestablished ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. As main conclusions had that Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
Keywords: Zika Virus
Genetic Markers
Whole Genome Sequencing
Virus Activation
Keywords in spanish: Virus Zika
Secuenciación Completa del Genoma
Activación Viral
keywords: Flavivirus
Genoma Viral
DeCS: Zika Virus
Marcadores Genéticos
Sequenciamento Completo do Genoma
Ativação Viral
Issue Date: 2019
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: STROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.
DOI: 10.1590/0074-02760190150
ISSN: 1678-8060
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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