Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35594
Title: Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
Authors: Silva, Roberta Peres da
Longo, Larissa G. V.
Cunha, Julia P. C. da
Sobreira, Tiago J. P.
Rodrigues, Marcio Lourenço
Faoro, Helisson
Goldenberg, Samuel
Alves, Lysangela Ronalte
Puccia, Rosana
Affilliation: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular -CeTICS. São Paulo, SP, Brasil.
Bindley Bioscience Center. Purdue University. West Lafayette, USA.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Abstract: Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.
Keywords: Extracellular Vesicles
Sequence Analysis
RNA
Messenger
Fungi
Keywords in spanish: Vesículas Extracelulares
Análisis de Secuencia de ARN
ARN Mensajero
Hongos
keywords: Paracoccidiodes
DeCS: Vesículas Extracelulares
Análise de Sequência de RNA
RNA Mensageiro
Fungos
Issue Date: 2019
Publisher: MDPI
Citation: SILVA, Roberta Peres da et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells, v. 8, n. 765, p. 1-21, 2019.
DOI: 10.3390/cells8070765
ISSN: 2073-4409
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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