Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35607
Title: Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis
Authors: Sousa, Rafaela Diniz
Kayano, Anderson Makoto
Caldeira, Cleópatra Alves da Silva
Silva, Rodrigo Simões
Monteiro, Marta C.
Dill, Leandro Soares Moreira
Grabner, Fernando P.
Calderon, Leonardo de Azevedo
Zuliani, Juliana Pavan
Stábeli, Rodrigo Guerino
Soares, Andreimar Martins
Affilliation: Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Experimental Biology. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Biodiversity and Biotechnology. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.
Federal University of Pará. School of Pharmacy. Belém, PA, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Fundação Oswaldo Cruz. Universidade Federal de São Carlos. Department of Medicine. University of São Paulo. Center of Translational Medicine and School of Medicine of Ribeirão Preto. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.
Abstract: Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and sequencing by Edman degradation. Results: The protein, called PocTX, was isolated using two chromatographic steps. Based on the phospholipase activity assay, electrophoresis and mass spectrometry, the protein presented a high degree of purity, with a mass of 13,896. 47 Da and a basic pI. After sequencing by the Edman degradation method, it was found that the protein showed a high identity with snake venom PLA2 homologues. Conclusion: This is the first report of an enzymatically inactive PLA2 isolated from wasp venom, similar to snake PLA2 homologues.
Keywords: Wasp
Polybia occidentalis
PocTX
PLA2 homologue
Issue Date: 2018
Publisher: Journal of Venomous Animals and Toxins including Tropical Diseases
Citation: SOUSA, Rafaela Diniz et al. Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. 1-6, 2018.
DOI: 10.1186/s40409-018-0143-1
ISSN: 1678-9199
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos




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