Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35642
Title: Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy
Authors: Caminha, Marcelle A.
Lorena, Virginia Maria B. de
Oliveira Júnior, Wilson de
Perales, Jonas
Carvalho, Paulo C.
Lima, Diogo B.
Cavalcanti, Maria da Glória A. M.
Martins, Sílvia M.
Valente, Richard H.
Menna-Barreto, Rubem F.S.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Universidade de Pernambuco. UPE-Pronto Socorro Cardiológico Universitário. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Espectrometria de Massa Computacional e Proteômica. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Espectrometria de Massa Computacional e Proteômica. Curitiba, PR, Brasil / Institut Pasteur. Mass Spectrometry for Biology Unit. Paris, France.
Universidade de Pernambuco. UPE-Pronto Socorro Cardiológico Universitário. Recife, PE, Brasil.
Universidade de Pernambuco. UPE-Pronto Socorro Cardiológico Universitário. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract: Chagas disease, caused by the protozoan Trypanosoma cruzi, affects millions of people worldwide, especially in Latin America. Approximately 30% of the cases evolve to the chronic symptomatic stage due to cardiac and/or digestive damage, generally accompanied by nervous system impairment. Given the higher frequency and severity of clinical manifestations related to cardiac tissue lesion, the goal of this study was the identification of proteins associated with the disease progression towards its cardiac form. Thus, T. cruzi bloodstream trypomastigotes proteins were submitted to immunoprecipitation using antibodies from patients with the asymptomatic or cardiac (stages B1 and C) forms of the disease and from healthy donors as control. Immunoreactive proteins were identified and quantified based on mass spectrometry analysis and shifts in the recognition profile were further evaluated. Compared to asymptomatic samples, IgG from stage C patients predominantly detected the I/6 autoantigen, whereas IgG from B1 patients resulted in higher yield of dihydrolipoamide acetyltransferase precursor, calpain cysteine peptidase, and two variants of CAP5.5. In this work, CAP5.5 recognition by serum immunoglobulin from patients with early cardiomyopathy generated a 23-fold abundance variation when compared to samples from asymptomatic patients, highlighting the participation of this protein in cardiac form progression of the disease. SIGNIFICANCE: While T. cruzi has become the major cause of infectious cardiomyopathy in Latin America, research groups have been struggling to find alternative treatment, vaccine candidates, and improved diagnostic tests. In addition, the absence of adequate biomarkers to assess cure and progression of disease is a major setback for clinical trials and patients monitoring. Therefore, our findings may contribute to a better understanding of T. cruzi pathogenesis and evaluation of suitable candidates for vaccine and diagnostic tests, besides the clinical applicability of the potential biomarkers for patient follow-up and prognosis. Finally, the identification of T. cruzi proteins recognized by IgG from healthy donors may contribute for the understanding and discovery of epitope conservation among a broad range of pathogens.
Keywords: Trypanosoma cruzi
Chagas Disease
Cardiomyopathy
Biomarkers
Mass spectrometry
CAP5.5
keywords: Trypanosoma cruzi
Doença de Chagas
Biomarcadores
Espectometria de massa
Cardiomiopatia
Issue Date: 2019
Publisher: Elsevier
Citation: CAMINHA, Marcelle A. et al. Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy. Journal of Proteomics, v. 194, p. 179-190, 2019.
DOI: 10.1016/j.jprot.2018.11.019
ISSN: 1874-3919
Copyright: restricted access
Appears in Collections:PR - ICC - Artigos de Periódicos
PE - IAM - Artigos de Periódicos
IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
MarcelleCaminha_VirginiaLorena_etal_IOC_2019.pdf1.43 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.