Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Fort Collins, CO, USA.
Faculdade de Medicina de São José do Rio Preto. São José do Rio Preto, SP, Brasil.
Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.
Mount Sinai School of Medicine. New York, NY, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil.
Massachusetts Institute of Technology. Cambridge, MA, USA.

Abstract

In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.

Keywords in Portuguese

Vírus do Nilo Ocidental
Teste de neutralização para redução de placa
Brasil

Keywords

WNV
b-ELISA
PRNT
Brazil

Publisher

Fundação Oswaldo Cruz. Instituto Oswaldo Cruz

Citation

MELANDRI, Vanessa et al. Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 8, p. 1073-1075, Dec. 2012.

DOI

10.1590/s0074-02762012000800020

ISSN

0074-0276

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35865

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